Prof. Dipl.-Ing. Dr. techn. Dr. med. habil. Harald Mischak

Prof. Dipl.-Ing. Dr. techn. Dr. med. habil. Harald Mischak

Harald Mischak, born 1961 St. Pölten in Austria, received his PhD in technical science from the Technical University of Vienna, Austria, in 1986. Between 1988 and 1993, after postdoctoral work on the Rhinovirus receptor at the University of Vienna (Institute for Biochemistry), he was on leave as an invited scientist on signalling by protein kinase C and Raf at the Laboratory of Viral Carcinogenesis (funded by the Fulbright Foundation) and as a Schroedinger and Fogarty Fellow at the Laboratory of Genetics of NIH National Cancer Institute in Bethesda, Maryland, USA.

He continued his research on kinases as Group Leader at the GSF - National Research Center for Environment and Health, Munich, Germany from 1993-1998. He wrote his habilitation in clinical microbiology at the Technische Universität München on Protein Kinase C in Signal Transduction. After one year as a scientific group leader at Franz-Volhard Klinikum (MDC) at Berlin-Buch, he worked on the structure of kinases and related molecules at the NIDDK, Bethesda, Maryland, USA. In 1999 he took up a position at the Department of Nephrology at Medical School of Hannover. Here he founded Mosaiques diagnostics and therapeutics AG in 2002, which was started with the aim to identify disease-specific polypeptides. Currently, he is the chief scientific officer of Mosaiques AG as well as executive director of Mosaiques diagnostics GmbH and Mosaiques DiaPat GmbH. With more than 400 scientific publications on signaling and proteomics that have been cited over 32.000 times (h-index 96 in Google), he is one of the leading experts worldwide in the field of proteome research, personalized medicine and applied systems biology. In addition, more than 100 patent applications have been filed with Prof. Mischak named as inventor, the majority on proteomic biomarkers.

Among his major achievements is the identification of distinct biological roles of Protein Kinase C. He was the first to show that Protein Kinase C isoforms display highly divergent biological properties, and he described these in differentiation and oncogenic transformation (Mischak et al. 1993, J Biol Chem 268, 1749-1756 and 20110-20115) and associated these with distinct intracellular localization (Goodnight, Mischak, et al. 1995, J Biol Chem 270:9991-10001). Together with Walter Kolch (for Raf signaling, e.g. Kolch et al. 1993, Nature 364:249-252) and Hans Hacker (for CpG signaling, e.g. Hacker, Mischak et al. 1998, EMBO J 17:6230-6240) he pinpointed the role of kinases in several major signaling mechanisms. All of these manuscripts have been cited several hundred times. Based on his experience on proteomics in basic research, he initiated the use of urinary proteomics and capillary electrophoresis coupled mass spectrometry for clinical application, and is the leading authority in clinical proteomics and biomarker identification. Among his achievement in this field are the development of guidelines for clinical proteome analysis, where he led a large international and multidisciplinary group to develop clinically relevant proteomic biomarkers (Mischak et al. 2007, Proteomics Clin Appl 1:148-156; 2010, Sci Transl Med 2:46ps42; 2012, Eur. J Clin Invest 42, 1027-1036), and the demonstration of successful application in the diagnosis and prognosis of several diseases (e.g. Decramer et al. 2006, Nat. Med. 12:398-400; Theodorescu et al. 2006, Lancet Oncol. 7:230-240; Rossing et al. 2008, JASN 19:1283-1290; Good et al. 2010, Molecular & Cellular Proteomics 9:2424-2437; Kuznetsova et al. 2012, Eur. Heart J. 33, 2342-2350).

The two main focuses of Prof Mischak’s work are:

  1. identification, validation, and implementation of proteomic biomarkers, aiming especially at biomarkers associated with chronic kidney disease, coronary artery disease, heart failure, and certain types of cancer
  2. uncovering the molecular changes on a proteomic level that are relevant in, or even cause of, the major diseases mentioned above. This approach is based on the biomarkers identified, but also on addition proteomic, metabolomic and genomic data. Using appropriate bioinformatic approaches, the high-dimensional data will be combined to identify the underlying molecular structures and ultimately develop a molecular model of the respective disease, which in turn will allow identifying the most appropriate therapeutic targets for intervention, and implementation of personalized precision medicine.

 

CURRICULUM VITAE:

Name: Harald Mischak
Date of Birth: 4.6.1961,      Nationality: Austrian
ORCID ID: 0000-0003-0323-0306
Current Post: Executive Director of Mosaiques Diagnostics GmbH, Chief Scientifc Officer of Mosaiques Diagnostics & Therapeutics AG
email: mischak@mosaiques-diagnostics.com
Telephon: (+49)-511-554744-13
Fax: (+49)-511-554744-31

 

Degrees & Honors:

Dipl.-Ing. (with distinction)   1984
Dr. techn. (with distinction)   1986
Dr. med. habil   1995

 

Education:

1971 - 1979 Realistisches Gymnasium (Grammar school with emphasis on science) in St. Pölten, Austria
1979 Matura with good Honors (school leaving certificate)
1979 - 1984 Department of Chemistry, Science Faculty Technical University of Vienna, Austria
1982 1. Diploma in Chemistry
1984 2. Diploma in Chemistry
1984 - 1986 Thesis at the University of Vienna, Subject: Immunotoxins
1986 - 1993 Post-Doc at the Institute for Biochemistry, University of Vienna (on leave from 1989-93), Austria
Sep.-Dec.1988 Invited scientist (funded by the Fulbright Foundation) to the Laboratory of Viral Carcinogenesis, NlH, NCI, FCRF, Frederick, Maryland, USA
1989 - 1990 Schroedinger Fellow at the Laboratory of Genetics, NIH, NCI, Bethesda, Maryland, USA
1990 - 1993 Fogarty Fellow at the Laboratory of Genetics, NIH, NCI, Bethesda, Maryland, USA
1993 - 1997 Scientific Group Leader (equivalent to assistant professor) at the Institute for Clinical Molecular Biology, GSF, Munich, Germany
1998 - 1998 Scientific Group Leader at the Technical University of Munich, Institute for Immunology, Munich, Germany
1998 - 1999 Scientific Group Leader at Franz-Volhard Klinikum, MDC, Berlin, Germany
1998 - 1999 Visiting Scientist (equivalent to guest professor) in the Laboratory of Molecular Biology, NIDDK, NIH, Bethesda, Maryland, USA
1999 - 2006 Professor, Medical School Hannover, Germany
2010 - 2017 Professor, BHF Glasgow Cardiovascular Research Centre University of Glasgow, UK
2017 - date Visiting Professor, BHF Glasgow Cardiovascular Research Centre University of Glasgow, UK
2002 - date Director of Mosaiques Diagnostics GmbH, Hannover, Germany

 

 Membership in Professional Societies:

Gesellschaft für Nephrologie 2004
EUTOX 2004
ERA-EDTA 2011

 

Editorial Work:

Senior Editor - Proteomics Clin. Appl. 2007
Section Editor - International Journal of Artificial Organs 2008
Academic Editor PLOS One 2013
Executive Advisory Board Proteomics 2014
Academic Editor Scientific Reports 2019

 

Membership in European Networks

PREDICTIONS 2004
INCA 2004
InGenious HyperCare 2006
Stemdiagnostics 2006
UROSYSTEOMICS 2006
GENINCA 2007
PREDIVA 2007
SysKID 2008
HEPACUTE 2009
EuroKUP 2009
ProtoClin 2010
PRIORITY 2011
MASCARA 2011
TrasnCyst 2012
CodeAge 2012
BCMolMed 2012
HOMAGE 2012
Frailomics 2012
BioMargin 2012
SysVasc 2013
CKD-BIO 2013
TransBioBC 2013
iMODE-CKD 2014
EURenOmics 2015
CaReSyAn 2017
ProACT 2017
PerstTIgAN 2018
Kidney Attack 2018
DCRen 2019
STRATEGY-CKD 2020

 

 

Bibliography

  1. Mischak,H., Kubicek,C.P., and Rohr,M. (1984). Citrate Inhibition of Glucose-Uptake in Aspergillus-Niger. Biotechnology Letters 6, 425-430.
  2. Kolar,H., Mischak,H., Kammel,W.P., and Kubicek,C.P. (1985). Carboxymethylcellulase and Beta-Glucosidase Secretion by Protoplasts of Trichoderma-Reesei. Journal of General Microbiology 131, 1339-1347.
  3. Meixner,O., Mischak,H., Kubicek,C.P., and Rohr,M. (1985). Effect of Manganese Deficiency on Plasma-Membrane Lipid-Composition and Glucose-Uptake in Aspergillus-Niger. Fems Microbiology Letters 26, 271-274.
  4. Mischak,H., Kubicek,C.P., and Rohr,M. (1985). Formation and Location of Glucose-Oxidase in Citric-Acid Producing Mycelia of Aspergillus-Niger. Applied Microbiology and Biotechnology 21, 27-31.
  5. Kowalski,H., Maurerfogy,I., Zorn,M., Mischak,H., Kuechler,E., and Blaas,D. (1987). Cleavage Site Between Vp1 and P2A of Human Rhinovirus Is Different in Serotype-2 and Serotype-14. Journal of General Virology 68, 3197-3200.
  6. Mischak,H., Neubauer,C., Berger,B., Kuechler,E., and Blaas,D. (1988). Detection of the Human Rhinovirus Minor Group Receptor on Renaturing Western Blots. Journal of General Virology 69, 2653-2656.
  7. Mischak,H., Neubauer,C., Kuechler,E., and Blaas,D. (1988). Characteristics of the Minor Group Receptor of Human Rhinoviruses. Virology 163, 19-25.
  8. Hofer,F., Weissinger,E., Mischak,H., Messner,R., Meixnermonori,B., Blaas,D., Visser,J., and Kubicek,C.P. (1989). A Monoclonal-Antibody Against the Alkaline Extracellular Beta-Glucosidase from Trichoderma-Reesei - Reactivity with Other Trichoderma Beta-Glucosidases. Biochimica et Biophysica Acta  992, 298-306.
  9. Mischak,H., Hofer,F., Messner,R., Weissinger,E., Hayn,M., Tomme,P., Esterbauer,H., Kuchler,E., Claeyssens,M., and Kubicek,C.P. (1989). Monoclonal-Antibodies Against Different Domains of Cellobiohydrolase I and Ii from Trichoderma-Reesei. Biochimica et Biophysica Acta 990, 1-7.
  10. Kolch,W., Weissinger,E., Mischak,H., Troppmair,J., Showalter,S.D., Lloyd,P., Heidecker,G., and Rapp,U.R. (1990). Probing Structure and Function of the Raf Protein-Kinase Domain with Monoclonal-Antibodies. Oncogene 5, 713-720.
  11. Largaespada,D., Kaehler,D., Weissinger,E., Mischak,H., Mushinski,F., and Risser,R. (1990). The Activity of An Abl-Myc Retrovirus in Fibroblast Cell-Lines and in Lymphocytes. Current Topics in Microbiology and Immunology 166, 91-96.
  12. Mischak,H., Kolch,W., Hofer,F., Weissinger,E., Gessl,A., Davidson,W.F., Aiello,F.B., Blaas,D., and Rapp,U.R. (1990). A Raf/Myc Virus Immortalized Macrophage Cell-Line Which Supports the Growth of B-Cell and B-Cell Hybridomas. Oncogene 5, 1377-1382.
  13. Weissinger,E.M., Largaespada,D., Smithgill,S.J., Risser,R., Mushinski,J.F., and Mischak,H. (1990). A Retrovirus Expressing V-Abl and C-Myc Induces Plasmacytomas in 100-Percent of Adult Pristane-Primed Balb/C Mice. Current Topics in Microbiology and Immunology 166, 121-127.
  14. Mischak,H., Kolch,W., Goodnight,J., Davidson,W.F., Rapp,U., Rosejohn,S., and Mushinski,J.F. (1991). Expression of Protein-Kinase-C Genes in Hematopoietic-Cells Is Cell-Type-Differentiation and B-Cell-Differentiation Stage Specific. Journal of Immunology 147, 3981-3987.
  15. Mischak,H., Bodenteich,A., Kolch,W., Goodnight,J., Hofer,F., and Mushinski,J.F. (1991). Mouse Protein-Kinase C-Delta, the Major Isoform Expressed in Mouse Hematopoietic-Cells - Sequence of the Cdna, Expression Patterns, and Characterization of the Protein. Biochemistry 30, 7925-7931.
  16. Weissinger,E.M., Mischak,H., Largaespada,D.A., Kaehler,D.A., Mitchell,T., Smithgill,S.J., Risser,R., and Mushinski,J.F. (1991). Induction of Plasmacytomas Secreting Antigen-Specific Monoclonal-Antibodies with A Retrovirus Expressing V-Abl and C-Myc. Proceedings of the National Academy of Sciences of the United States of America 88, 8735-8739.
  17. Dlugosz,A.A., Mischak,H., Mushinski,J.F., and Yuspa,S.H. (1992). Transcripts Encoding Protein Kinase-C-Alpha, Kinase-C-Delta, Kinase-C, Kinase-C-Zeta, and Kinase-C-Eta Are Expressed in Basal and Differentiating Mouse Keratinocytes Invitro and Exhibit Quantitative Changes in Neoplastic-Cells. Molecular Carcinogenesis 5, 286-292.
  18. Goodnight,J., Kazanietz,M.G., Blumberg,P.M., Mushinski,J.F., and Mischak,H. (1992). The cDNA sequence, expression pattern and protein characteristics of mouse protein kinase C-zeta. Gene 122, 305-311.
  19. Largaespada, D., Kaehler, D., Mischak, H., Weissinger, E., Poster, D., Mushinsky, J. F., and Risser, R. A retrovirus that express v-abl and c-myc oncogenes rapidly induces plasmacytomas. Oncogene 7[4], 811-819. 1992.
    Ref Type: Journal (Full)
  20. Arnold,T.P., Standaert,M.L., Hernandez,H., Watson,J., Mischak,H., Kazanietz,M.G., Zhao,L.M., Cooper,D.R., and Farese,R.V. (1993). Effects of Insulin and Phorbol Esters on Marcks (Myristoylated Alanine-Rich C-Kinase Substrate) Phosphorylation (and Other Parameters of Protein-Kinase-C Activation) in Rat Adipocytes, Rat Soleus Muscle and Bc3H-1 Myocytes. Biochemical Journal 295, 155-164.
  21. Kazanietz,M.G., Areces,L.B., Bahador,A., Mischak,H., Goodnight,J., Mushinski,J.F., and Blumberg,P.M. (1993). Characterization of Ligand and Substrate-Specificity for the Calcium-Dependent and Calcium-Independent Protein-Kinase-C Isozymes. Molecular Pharmacology 44, 298-307.
  22. Kolch,W., Heidecker,G., Kochs,G., Hummel,R., Vahidi,H., Mischak,H., Finkenzeller,G., Marme,D., and Rapp,U.R. (1993). Protein Kinase-C-Alpha Activates Raf-1 by Direct Phosphorylation. Nature 364, 249-252.
  23. Martiny-Baron,G., Kazanietz,M.G., Mischak,H., Blumberg,P.M., Kochs,G., Hug,H., Marme,D., and Schachtele,C. (1993). Selective inhibition of protein kinase C isozymes by the indolocarbazole Go 6976. J Biol. Chem. 268, 9194-9197.
  24. Mischak,H., Pierce,J.H., Goodnight,J., Kazanietz,M.G., Blumberg,P.M., and Mushinski,J.F. (1993). Phorbol Ester-Induced Myeloid Differentiation Is Mediated by Protein Kinase-C-Alpha and Kinase-C-Delta and Not by Protein Kinase-C-Beta-Ii, Kinase-C-Delta, Kinase-C-Zeta, and Kinase-C-Eta. Journal of Biological Chemistry 268, 20110-20115.
  25. Mischak,H., Goodnight,J., Henderson,D.W., Osada,S., Ohno,S., and Mushinski,J.F. (1993). Unique Expression Pattern of Protein-Kinase-C -Theta - High Messenger-Rna Levels in Normal Mouse Testes and in T-Lymphocytic Cells and Neoplasms. Febs Letters 326, 51-55.
  26. Mischak,H., Goodnight,J., Kolch,W., Martinybaron,G., Schaechtle,C., Kazanietz,M.G., Blumberg,P.M., Pierce,J.H., and Mushinski,J.F. (1993). Overexpression of Protein Kinase-C-Delta and Kinase-Epsilon in Nih 3T3 Cells Induces Opposite Effects on Growth, Morphology, Anchorage Dependence, and Tumorigenicity. Journal of Biological Chemistry 268, 6090-6096.
  27. Ozawa,K., Szallasi,Z., Kazanietz,M.G., Blumberg,P.M., Mischak,H., Mushinski,J.F., and Beaven,M.A. (1993). Ca(2+)-dependent and Ca(2+)-independent isozymes of protein kinase C mediate exocytosis in antigen-stimulated rat basophilic RBL-2H3 cells. Reconstitution of secretory responses with Ca2+ and purified isozymes in washed permeabilized cells. J Biol. Chem. 268 , 1749-1756.
  28. Weissinger,E.M., Mischak,H., Goodnight,J., Davidson,W.F., and Mushinski,J.F. (1993). Addition of Constitutive C-Myc Expression to Abelson Murine Leukemia-Virus Changes the Phenotype of the Cells Transformed by the Virus from Pre-B-Cell Lymphomas to Plasmacytomas. Molecular and Cellular Biology 13, 2578-2585.
  29. Eissner,G., Kolch,W., Mischak,H., Bornkamm,G.W., and Holler,E. (1994). Differential Role of Protein-Kinase-C in Cytokine-Induced Lymphocyte-Endothelium Interaction In-Vitro. Scandinavian Journal of Immunology 40, 395-402.
  30. Farese,R.V., Standaert,M.L., Arnold,T.P., Yamada,K., Musunuru,K., Hernandez,H., Mischak,H., and Cooper,D.R. (1994). Preferential Activation of Microsomal Diacylglycerol/Protein Kinase-C Signaling During Glucose Treatment (De-Novo Phospholipid-Synthesis) of Rat Adipocytes. Journal of Clinical Investigation 93, 1894-1899.
  31. Goodnight,J., Mischak,H., and Mushinski,J.F. (1994). Association of Elevated Levels of Protein-Kinase C-Zeta Messenger-Rna and Protein with Murine B-Lymphocytic Neoplasia. Molecular Carcinogenesis 11, 131-137.
  32. Goodnight,J., Mischak,H., and Mushinski,J.F. (1994). Selective Involvement of Protein-Kinase-C Isozymes in Differentiation and Neoplastic Transformation. Advances in Cancer Research, Vol 64 64, 159-209.
  33. Hafner,S., Adler,H.S., Mischak,H., Janosch,P., Heidecker,G., Wolfman,A., Pippig,S., Lohse,M., Ueffing,M., and Kolch,W. (1994). Mechanism of Inhibition of Raf-1 by Protein-Kinase-A. Molecular and Cellular Biology 14, 6696-6703.
  34. Huppi,K., Siwarski,D., Goodnight,J., and Mischak,H. (1994). Assignment of the Protein-Kinase-C-Delta Polypeptide Gene (Prkcd) to Human-Chromosome-3 and Mouse Chromosome-14. Genomics 19, 161-162.
  35. Kazanietz,M.G., Bustelo,X.R., Barbacid,M., Kolch,W., Mischak,H., Wong,G., Pettit,G.R., Bruns,J.D., and Blumberg,P.M. (1994). Zinc-Finger Domains and Phorbol Ester Pharmacophore - Analysis of Binding to Mutated Form of Protein Kinase-C-Tau and the Vav and C-Raf Protooncogene Products. Journal of Biological Chemistry 269, 11590-11594.
  36. Lehel,C., Olah,Z., Mischak,H., Mushinski,J.F., and Anderson,W.B. (1994). Overexpressed Protein-Kinase C-Delta and C-Epsilon Subtypes in Nih 3T3 Cells Exhibit Differential Subcellular-Localization and Differential Regulation of Sodium-Dependent Phosphate-Uptake. Journal of Biological Chemistry 269, 4761-4766.
  37. Li,W.Q., Mischak,H., Yu,J.C., Wang,L.M., Mushinski,J.F., Heidaran,M.A., and Pierce,J.H. (1994). Tyrosine Phosphorylation of Protein-Kinase C-Delta in Response to Its Activation. Journal of Biological Chemistry 269, 2349-2352.
  38. Morawetz,R., Mischak,H., Goodnight,J., Lendenfeld,T., Mushinsky,J.F., and Kubicek,C.P. (1994). A Protein Kinase-Encoding Gene, Pkt1, from Trichoderma-Reesei, Homologous to the Yeast Ypk1 and Ypk2 (Ykr2) Genes. Gene 146, 309-310.
  39. Weissinger,E.M., Henderson,D.W., Mischak,H., Goodnight,J., and Mushinski,J.F. (1994). Induction of Plasmacytomas That Secrete Monoclonal Antipeptide Antibodies by Retroviral Transformation. Journal of Immunological Methods 168, 123-130.
  40. Yamada,K., Standaert,M.L., Yu,B., Mischak,H., Cooper,D.R., and Farese,R.V. (1994). Insulin-Like Effects of Sodium Orthovanadate on Diacylglycerol-Protein Kinase-C Signaling in Bc3H-1 Myocytes. Archives of Biochemistry and Biophysics 312, 167-172.
  41. Avignon,A., Standaert,M.L., Yamada,K., Mischak,H., Spencer,B., and Farese,R.V. (1995). Insulin Increases Messenger-Rna Levels of Protein-Kinase-C-Alpha and Protein-Kinase-C-Beta in Rat Adipocytes and Protein-Kinase-C-Alpha, Protein-Kinase-C-Beta and Protein-Kinase-C-Theta in Rat Skeletal-Muscle. Biochemical Journal 308, 181-187.
  42. Avignon,A., Standaert,M.L., Yamada,K., Mischak,H., Spencer,B., and Farese,R.V. (1995). Insulin increases mRNA levels of protein kinase C-alpha and -beta in rat adipocytes and protein kinase C-alpha, -beta and -theta in rat skeletal muscle. Biochem. J 308 ( Pt 1), 181-187.
  43. Chalfant,C.E., Mischak,H., Watson,J.E., Winkler,B.C., Goodnight,J., Farese,R.V., and Cooper,D.R. (1995). Regulation of Alternative Splicing of Protein-Kinase C-Beta by Insulin. Journal of Biological Chemistry 270, 13326-13332.
  44. Crespo,P., Mischak,H., and Gutkind,J.S. (1995). Overexpression of Mammalian Protein-Kinase C-Zeta Does Not Affect the Growth-Characteristics of Nih 3T3 Cells. Biochemical and Biophysical Research Communications 213, 266-272.
  45. Goodnight,J.A., Mischak,H., Kolch,W., and Mushinski,J.F. (1995). Immunocytochemical localization of eight protein kinase C isozymes overexpressed in NIH 3T3 fibroblasts. Isoform-specific association with microfilaments, Golgi, endoplasmic reticulum, and nuclear and cell membranes. J Biol. Chem.  270, 9991-10001.
  46. Kieser,A., Goodnight,J., Kolch,W., Mischak,H., and Mushinski,J.F. (1995). Identification of the Primary Growth-Response Gene, St2/T1, As A Gene Whose Expression Is Differentially Regulated by Different Protein-Kinase-C Isozymes. Febs Letters 372, 189-193.
  47. Li,S.F., Janosch,P., Tanji,M., Rosenfeld,G.C., Waymire,J.C., Mischak,H., Kolch,W., and Sedivy,J.M. (1995). Regulation of Raf-1 Kinase-Activity by the 14-3-3-Family of Proteins. Embo Journal 14, 685-696.
  48. Janosch,P., Schellerer,M., Seitz,T., Reim,P., Eulitz,M., Brielmeier,M., Kolch,W., Sedivy,J.M., and Mischak,H. (1996). Characterization of I kappa B kinases - I kappa B-alpha is not phosphorylated by Raf-1 or protein kinase C isozymes, but is a casein kinase II substrate. Journal of Biological Chemistry 271, 13868-13874.
  49. Kieser,A., Seitz,T., Adler,H.S., Coffer,P., Kremmer,E., Crespo,P., Gutkind,J.S., Henderson,D.W., Mushinski,J.F., Kolch,W., and Mischak,H. (1996). Protein kinase C-zeta reverts v-raf transformation of NIH-3T3 cells. Genes & Development 10, 1455-1466.
  50. Kolch,W., Philipp,A., Mischak,H., Dutil,E.M., Mullen,T.M., Feramisco,J.R., Meinkoth,J.L., and Rose,D.W. (1996). Inhibition of Raf-1 signaling by a monoclonal antibody, which interferes with Raf-1 activation and with Mek substrate binding. Oncogene 13, 1305-1314.
  51. Mischak,H., Seitz,T., Janosch,P., Eulitz,M., Steen,H., Schellerer,M., Philipp,A., and Kolch,W. (1996). Negative regulation of Raf-1 by phosphorylation of serine 621. Molecular and Cellular Biology 16, 5409-5418.
  52. Morawetz,R., Lendenfeld,T., Mischak,H., Muhlbauer,M., Gruber,F., Goodnight,J., deGraaff,L.H., Visser,J., Mushinski,J.F., and Kubicek,C.P. (1996). Cloning and characterisation of genes (pkc1 and pkcA) encoding protein kinase C homologues from Trichoderma reesei and Aspergillus niger. Molecular & General Genetics 250, 17-28.
  53. Perletti,G.P., Folini,M., Lin,H.C., Mischak,H., Piccinini,F., and Tashjian,A.H. (1996). Overexpression of protein kinase C epsilon is oncogenic in rat colonic epithelial cells. Oncogene 12, 847-854.
  54. Bossenmaier,B., Mosthaf,L., Mischak,H., Ullrich,A., and Haring,H.U. (1997). Protein kinase C isoforms beta 1 and beta 2 inhibit the tyrosine kinase activity of the insulin receptor. Diabetologia 40, 863-866.
  55. Kellerer,M., Mushack,J., Mischak,H., and Haring,H.U. (1997). Protein kinase C (PKC) epsilon enhances the inhibitory effect of TNF alpha on insulin signaling in HEK293 cells. Febs Letters 418, 119-122.
  56. Ueffing,M., Lovric,J., Philipp,A., Mischak,H., and Kolch,W. (1997). Protein kinase C-epsilon associates with the Raf-1 kinase and induces the production of growth factors that stimulate Raf-1 activity. Oncogene 15, 2921-2927.
  57. Wang,Q.J., Acs,P., Goodnight,J., Giese,T., Blumberg,P.M., Mischak,H., and Mushinski,J.F. (1997). The catalytic domain of protein kinase C-delta in reciprocal delta and epsilon chimeras mediates phorbol ester-induced macrophage differentiation of mouse promyelocytes. J Biol. Chem. 272 , 76-82.
  58. Weissinger,E.M., Eissner,G., Grammer,C., Fackler,S., Haefner,B., Yoon,L.S., Lu,K.S., Bazarov,A., Sedivy,J.M., Mischak,H., and Kolch,W. (1997). Inhibition of the Raf-1 kinase by cyclic AMP agonists causes apoptosis of v-abl-transformed cells. Molecular and Cellular Biology 17, 3229-3241.
  59. Baumann,M., Mischak,H., Dammeier,S., Kolch,W., Gires,O., Pich,D., Zeidler,R., Delecluse,H.J., and Hammerschmidt,W. (1998). Activation of the Epstein-Barr virus transcription factor BZLF1 by 12-O-tetradecanoylphorbol-13-acetate-induced phosphorylation. Journal of Virology 72, 8105-8114.
  60. Hacker,H., Mischak,H., Miethke,T., Liptay,S., Schmid,R., Sparwasser,T., Heeg,K., Lipford,G.B., and Wagner,H. (1998). CpG-DNA-specific activation of antigen-presenting cells requires stress kinase activity and is preceded by non-specific endocytosis and endosomal maturation. Embo Journal 17, 6230-6240.
  61. Kellerer,M., Mushack,J., Seffer,E., Mischak,H., Ullrich,A., and Haring,H.U. (1998). Protein kinase C isoforms alpha, delta and theta require insulin receptor substrate-1 to inhibit the tyrosine kinase activity of the insulin receptor in human kidney embryonic cells (HEK 293 cells). Diabetologia  41, 833-838.
  62. Lovric,J., Dammeier,S., Kieser,A., Mischak,H., and Kolch,W. (1998). Activated Raf induces the hyperphosphorylation of stathmin and the reorganization of the microtubule network. Journal of Biological Chemistry 273, 22848-22855.
  63. Maucher,C., Weissinger,E.M., Kremmer,E., Baccarini,M., Procyk,K., Henderson,D.W., Wolff,L., Kolch,W., Kaspers,B., Mushinski,J.F., and Mischak,H. (1998). Activation of bcl-2 suppressible 40 and 44 kDa p38-like kinases during apoptosis of early and late B lymphocytic cell lines. Febs Letters 427, 29-35.
  64. Muller,G., Storz,P., Bourteele,S., Doppler,H., Pfizenmaier,K., Mischak,H., Philipp,A., Kaiser,C., and Kolch,W. (1998). Regulation of Raf-1 kinase by TNF via its second messenger ceramide and cross-talk with mitogenic signalling. Embo Journal 17, 732-742.
  65. Prouty,S.M., Maroo,A., Maucher,C., Mischak,H., Kolch,W., and Sedivy,J.M. (1998). Studies of perinuclear and nuclear translocation of the Raf-1 protein in rodent fibroblasts. Biochimica et Biophysica Acta-Molecular Cell Research 1402, 6-16.
  66. Wang,Q.J., Acs,P., Goodnight,J.A., Blumberg,P.M., Mischak,H., and Mushinski,J.F. (1998). The catalytic domain of PKC-epsilon, in reciprocal PKC-delta and -epsilon chimeras, is responsible for conferring tumorgenicity to NIH3T3 cells, whereas both regulatory and catalytic domains of PKC-epsilon contribute to in vitro transformation. Oncogene 16, 53-60.
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  79. Brandt,D., Gimona,M., Hillmann,M., Haller,H., and Mischak,H. (2002). Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway. Journal of Biological Chemistry 277, 20903-20910.
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  82. Hennige,A.M., Fritsche,A., Strack,V., Weigert,C., Mischak,H., Borboni,P., Renn,W., Haring,H.U., and Kellerer,M. (2002). PKC zeta enhances insulin-like growth factor 1-dependent mitogenic activity in the rat clonal beta cell line RIN 1046-38. Biochemical and Biophysical Research Communications 290, 85-90.
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  84. Brandt,D.T., Goerke,A., Heuer,M., Gimona,M., Leitges,M., Kremmer,E., Lammers,R., Haller,H., and Mischak,H. (2003). Protein kinase C delta induces Src kinase activity via activation of the protein tyrosine phosphatase PTP alpha. Journal of Biological Chemistry 278, 34073-34078.
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  93. Kaiser,T., Kamal,H., Rank,A., Kolb,H.J., Holler,E., Ganser,A., Hertenstein,B., Mischak,H., and Weissinger,E.M. (2004). Proteomics applied to the clinical follow-up of patients after allogeneic hematopoietic stem cell transplantation. Blood 104, 340-349.
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  99. Weissinger, E. M. and Mischak, H. Proteom analysis applied towards early diagnosis of renal diseases and transplant-monitoring. Transplantationsmedizin 16, 2-9. 2004.
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  107. Kolch,W., Mischak,H., and Pitt,A.R. (2005). The molecular make-up of a tumour: proteomics in cancer research. Clinical Science 108, 369-383.
  108. Kolch,W., Neususs,C., Peizing,M., and Mischak,H. (2005). Capillary electrophoresis - Mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery. Mass Spectrometry Reviews 24, 959-977.
  109. Meier,M., Kaiser,T., Herrmann,A., Knueppel,S., Hillmann,M., Koester,P., Danne,T., Haller,H., Fliser,D., and Mischak,H. (2005). Identification of urinary protein pattern in Type 1 diabetic adolescents with early diabetic nephropathy by a novel combined proteome analysis. Journal of Diabetes and Its Complications 19, 223-232.
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  112. Theodorescu,D., Fliser,D., Wittke,S., Mischak,H., Krebs,R., Walden,M., Ross,M., Eltze,E., Bettendorf,O., Wulfing,C., and Semjonow,A. (2005). Pilot study of capillary electrophoresis coupled to mass spectrometry as a tool to define potential prostate cancer biomarkers in urine. Electrophoresis 26, 2797-2808.
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  114. Wittke,S., Haubitz,M., Walden,M., Rohde,F., Schwarz,A., Mengel,M., Mischak,H., Haller,H., and Gwinner,W. (2005). Detection of acute tubulointerstitial rejection by proteomic analysis of urinary samples in renal transplant recipients. American Journal of Transplantation 5, 2479-2488.
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  116. Decramer,S., Wittke,S., Mischak,H., Zurbig,P., Walden,M., Bouissou,F., Bascands,J.L., and Schanstra,J.P. (2006). Predicting the clinical outcome of congenital unilateral ureteropelvic junction obstruction in newborn by urinary proteome analysis. Nature Medicine 12, 398-400.
  117. Girolami, M., Mischak, H., and Krebs, R. Analysis of Complex, Multi-Dimensional Data Sets. Drug Discovery Today 3, 13-19. 2006.
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  119. Theodorescu,D., Wittke,S., Ross,M.M., Walden,M., Conaway,M., Just,I., Mischak,H., and Frierson,H.F. (2006). Discovery and validation of new protein biomarkers for 4 urothelial cancer: a prospective analysis. Lancet Oncology 7, 230-240.
  120. Weissinger,E.M., Mischak,H., Ganser,A., and Hertenstein,B. (2006). Value of proteomics applied to the follow-up in stem cell transplantation. Annals of Hematology 85, 205-211.
  121. Weissinger,E.M., Nguyen-Khoa,T., Fumeron,C., Saltiel,C., Walden,M., Kaiser,T., Mischak,H., Drueke,T.B., Lacour,B., and Massy,Z.A. (2006). Effects of oral vitamin C supplementation in hemodialysis patients: A proteomic assessment. Proteomics 6, 993-1000.
  122. Zurbig,P., Renfrow,M.B., Schiffer,E., Novak,J., Walden,M., Wittke,S., Just,I., Pelzing,M., Neususs,C., Theodorescu,D., Root,K.E., Ross,M.M., and Mischak,H. (2006). Biomarker discovery by CE-MS enables sequence analysis via MS/MS with platform-independent separation. Electrophoresis 27, 2111-2125.
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  127. Julian,B.A., Wittke,S., Novak,J., Good,D.M., Coon,J.J., Kellmann,M., Zurbig,P., Schiffer,E., Haubitz,M., Moldoveanu,Z., Calcatera,S.M., Wyatt,R.J., Sykora,J., Sladkova,E., Hes,O., Mischak,H., and McGuire,B.M. (2007). Electrophoretic methods for analysis of urinary polypeptides in IgA-associated renal diseases. Electrophoresis 28, 4469-4483.
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  136. Jiang,H., Schiffer,E., Song,Z.F., Wang,J.W., Zurbig,P., Thedieck,K., Moes,S., Bantel,H., Saal,N., Jantos,J., Brecht,M., Jeno,P., Hall,M.N., Hager,K., Manns,M.P., Hecker,H., Ganser,A., Dohner,K., Bartke,A., Meissner,C., Mischak,H., Ju,Z.Y., and Rudolph,K.L. (2008). Proteins induced by telomere dysfunction and DNA damage represent biomarkers of human aging and disease. Proceedings of the National Academy of Sciences of the United States of America 105, 11299-11304.
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  138. Raedler,T.J., Wittke,S., Jahn,H., Koessler,A., Mischak,H., and Wiedemann,K. (2008). Capillary electrophoresis mass spectrometry as a potential tool to detect lithium-induced nephropathy: Preliminary results. Progress in Neuro-Psychopharmacology & Biological Psychiatry 32, 673-678.
  139. Rossing,K., Mischak,H., Rossing,P., Schanstra,J.P., Wiseman,A., and Maahs,D.M. (2008). The urinary proteome in diabetes and diabetes-associated complications: New ways to assess disease progression and evaluate therapy. Proteomics Clinical Applications 2, 997-1007.
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  142. Schiffer,E., Mischak,H., Theodorescu,D., and Vlahou,A. (2008). Challenges of using mass spectrometry as a bladder cancer biomarker discovery platform. World Journal of Urology 26, 67-74.
  143. Theodorescu,D., Schiffer,E., Bauer,H.W., Douwes,F., Eichhorn,F., Polley,R., Schmidt,T., Schofer,W., Zurbig,P., Good,D.M., Coon,J.J., and Mischak,H. (2008). Discovery and validation of urinary biomarkers for propstate cancer. Proteomics Clinical Applications 2, 556-570.
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