Prof. Dipl.-Ing. Dr. techn. Dr. med. habil. Harald Mischak

Prof. Dipl.-Ing. Dr.techn. Dr.med.habil. Harald Mischak

Harald Mischak, born 1961 St. Pölten in Austria, received his PhD in technical science from the Technical University of Vienna, Austria, in 1986 (grade “excellent”).

Between 1988 and 1993, after postdoctoral work on the Rhinovirus receptor at the University of Vienna (Institute for Biochemistry), he was on leave as an invited scientist on signalling by protein kinase C and Raf at the Laboratory of Viral Carcinogenesis (funded by the Fulbright Foundation) and as a Schroedinger and Fogarty Fellow at the Laboratory of Genetics of NIH National Cancer Institute in Bethesda, Maryland, USA.

He continued his research on kinases as Group Leader at the GSF - National Research Center for Environment and Health, Munich, Germany from 1993-1998. He wrote his habilitation in clinical microbiology at the Technische Universität München on Protein Kinase C in Signal Transduction. After one year as a scientific group leader at Franz-Volhard Klinikum (MDC) at Berlin-Buch, he worked on the structure of kinases and related molecules at the NIDDK, Bethesda, Maryland, USA. In 1999 he took up a position at the Department of Nephrology at Medical School of Hannover. Here he founded Mosaiques diagnostics and therapeutics AG in 2002, which was started with the aim to identify disease-specific polypeptides. Currently, he holds a position as Professor for Proteomics and Systems Medicine at the University of Glasgow, and he is the chief scientific officer of Mosaiques AG as well as executive director of Mosaiques diagnostics GmbH and Mosaiques DiaPat GmbH. With more than 300 scientific publications on signaling and proteomics that have been cited over 20000 times (h-index 82 in Google, 74 in Web-of-Science), he is one of the leading experts worldwide in the field of proteome research and applied systems biology. In addition, more than 100 patent applications have been filed with Prof. Mischak named as inventor, the majority on proteomic biomarkers.

 

Among his major achievements is the identification of distinct biological roles of Protein Kinase C. He was the first to show that Protein Kinase C isoforms display highly divergent biological properties, and he described these in differentiation and oncogenic transformation (Mischak et al. 1993, J Biol Chem 268, 1749-1756 and 20110-20115) and associated these with distinct intracellular localization (Goodnight, Mischak, et al. 1995, J Biol Chem 270:9991-10001). Together with Walter Kolch (for Raf signaling, e.g. Kolch et al. 1993, Nature 364:249-252) and Hans Hacker (for CpG signaling, e.g. Hacker, Mischak et al. 1998, EMBO J 17:6230-6240) he pinpointed the role of kinases in several major signaling mechanisms. All of these manuscripts have been cited several hundred times. Based on his experience on proteomics in basic research, he initiated the use of urinary proteomics and capillary electrophoresis coupled mass spectrometry for clinical application, and is the leading authority in clinical proteomics and biomarker identification. Among his achievement in this field are the development of guidelines for clinical proteome analysis, where he led a large international and multidisciplinary group to develop clinically relevant proteomic biomarkers (Mischak et al. 2007, Proteomics Clin Appl 1:148-156; 2010, Sci Transl Med 2:46ps42; 2012, Eur. J Clin Invest 42, 1027-1036), and the demonstration of successful application in the diagnosis and prognosis of several diseases (e.g. Decramer et al. 2006, Nat. Med. 12:398-400; Theodorescu et al. 2006, Lancet Oncol. 7:230-240; Rossing et al. 2008, JASN 19:1283-1290; Good et al. 2010, Molecular & Cellular Proteomics 9:2424-2437; Kuznetsova et al. 2012, Eur. Heart J. 33, 2342-2350).

The two main focuses of Prof Mischak’s work are:

1)      identification, validation, and implementation of proteomic biomarkers, aiming especially at biomarkers associated with chronic kidney disease, coronary artery disease, heart failure, and certain types of cancer

2)      uncovering the molecular changes on a proteomic level that are relevant in, or even cause of, the major diseases mentioned above. This approach is based on the biomarkers identified, but also on addition proteomic, metabolomic and genomic data. Using appropriate bioinformatic approaches, the high-dimensional data will be combined to identify the underlying molecular structures and ultimately develop a molecular model of the respective disease, which in turn will allow identifying the most appropriate therapeutic targets for intervention.

 

CURRICULUM VITAE:

Name: Harald Mischak
Date of Birth: 4.6.1961,      Nationality: Austrian
ORCID ID: 0000-0003-0323-0306
Current Post: Executive Director of Mosaiques Diagnostics GmbH, Chief Scientifc Officer of Mosaiques Diagnostics & Therapeutics AG
email: mischak@mosaiques-diagnostics.com
Telephon: (+49)-511-554744-13
Fax: (+49)-511-554744-31
   
and Full Professor and Chair of Proteomics and Systems Medicine
  University of Glasgow
  126 University Avenue,
  Glasgow, G12 8TA
email: h.mischak@clinmed.gla.ac.uk
Tel: +44(0)141 330 3325 or 6210
Fax: +44(0)141 330 6972

 

Degrees & Honors:

Dipl.Ing (with distinction)   1984
Dipl.Ing (with distinction)   1986
Dr.med.habil   1995

 

Education:

1971 - 1979: Realistisches Gymnasium (GrammarSchool with emphasis on science) in St.Poelten, Austria
1979: Matura with good Honors (School leaving certificate)
1979 - 1984: Department of Chemistry ,Science Faculty Technical University of Vienna
1982: 1.Diploma in Chemistry
1984: 2.Diploma  in Chemistry
1984 - 1986: Thesis at the University of Vienna.Subject: Immunotoxins
1986 - 1993: Hochschul-Assistent at the Institute for Biochemistry, University of Vienna (on leave from 1989-93)
Sep.-Dec.1988: Invited scientist (funded by the Fulbright Foundation) to the Laboratory of Viral Carcinogenesis,NlH,NCI, FCRF, Frederick, Maryland, USA
1989 - 1990: Schroedinger Fellow at the Lab. of Genetics, NIH, NCI, Bethesda, Maryland, USA
1990 - 1993: Fogarty Fellow at the Lab. of Genetics, NIH,NCI.
1993 -1997: Scientific Group Leader (equivalent to assistant professor) at the Inst. f. Klin. Mol. Biol., GSF, Munich, Germany
1998 - 1998: Scientific Group Leader at the Technische Universität München, Institute for Immunologie
1998 - 1999: Scientific Group Leader at Franz-Volhard Klinikum, MDC, Berlin ´Buch.
1998 - 1999: Visiting Scientist (equivalent to guest professor) in the Laboratory of Molecular Biology, NIDDK, NIH, Bethesda (Maryland, USA).
1999 - 2006: Professor, MH Hannover, GERMANY
2002 - date: Director of Mosaiques Diagnostics GmbH
2010 – date: Full Professor, BHF Glasgow Cardiovascular Research Centre University of Glasgow, UK

 

 Membership in Professional Societies:

Deutsche Krebsgesellschaft 2002
Gesellschaft für Nephrologie 2004
Gesellschaft für Proteomforschung 2006
EUTOX 2004
ERA EDTA 2011

 

Editorial Work:

Section Editor - International Journal of Artificial Organs 2008
Senior Editor - Proteomics Clin Appl 2007
Academic Editor PLOS One 2013

 

Membership in European Networks

PREDICTIONS 2004
INCA 2004
InGenious HyperCare 2006
Stemdiagnostics 2006
UROSYSTEOMICS 2006
GENINCA 2007
PREDIVA 2007
SysKID 2008
HEPACUTE 2009
EuroKUP 2009
ProtoClin 2010
PRIORITY 2011
MASCARA 2011
TrasnCyst 2012
CodeAge 2012
BCMolMed 2012
HOMAGE 2012
Frailomics 2012
BioMargin 2012
SysVasc 2013
CKD-BIO 2013
TransBioBC 2013
iMODE-CKD 2014
EuRenomics 2015

 

Bibliography

1. Mischak,H., Kubicek,C.P., and Rohr,M. (1984). Citrate Inhibition of Glucose-Uptake in Aspergillus-Niger. Biotechnology Letters 6, 425-430.

2. Kolar,H., Mischak,H., Kammel,W.P., and Kubicek,C.P. (1985). Carboxymethylcellulase and Beta-Glucosidase Secretion by Protoplasts of Trichoderma-Reesei. Journal of General Microbiology 131, 1339-1347.

3. Meixner,O., Mischak,H., Kubicek,C.P., and Rohr,M. (1985). Effect of Manganese Deficiency on Plasma-Membrane Lipid-Composition and Glucose-Uptake in Aspergillus-Niger. Fems Microbiology Letters 26, 271-274.

4. Mischak,H., Kubicek,C.P., and Rohr,M. (1985). Formation and Location of Glucose-Oxidase in Citric-Acid Producing Mycelia of Aspergillus-Niger. Applied Microbiology and Biotechnology 21, 27-31.

5. Kowalski,H., Maurerfogy,I., Zorn,M., Mischak,H., Kuechler,E., and Blaas,D. (1987). Cleavage Site Between Vp1 and P2A of Human Rhinovirus Is Different in Serotype-2 and Serotype-14. Journal of General Virology 68, 3197-3200.

6. Mischak,H., Neubauer,C., Berger,B., Kuechler,E., and Blaas,D. (1988). Detection of the Human Rhinovirus Minor Group Receptor on Renaturing Western Blots. Journal of General Virology 69, 2653-2656.

7. Mischak,H., Neubauer,C., Kuechler,E., and Blaas,D. (1988). Characteristics of the Minor Group Receptor of Human Rhinoviruses. Virology 163, 19-25.

8. Hofer,F., Weissinger,E., Mischak,H., Messner,R., Meixnermonori,B., Blaas,D., Visser,J., and Kubicek,C.P. (1989). A Monoclonal-Antibody Against the Alkaline Extracellular Beta-Glucosidase from Trichoderma-Reesei - Reactivity with Other Trichoderma Beta-Glucosidases. Biochimica et Biophysica Acta  992, 298-306.

9. Mischak,H., Hofer,F., Messner,R., Weissinger,E., Hayn,M., Tomme,P., Esterbauer,H., Kuchler,E., Claeyssens,M., and Kubicek,C.P. (1989). Monoclonal-Antibodies Against Different Domains of Cellobiohydrolase I and Ii from Trichoderma-Reesei. Biochimica et Biophysica Acta 990, 1-7.

10.  Kolch,W., Weissinger,E., Mischak,H., Troppmair,J., Showalter,S.D., Lloyd,P., Heidecker,G., and Rapp,U.R. (1990). Probing Structure and Function of the Raf Protein-Kinase Domain with Monoclonal-Antibodies. Oncogene 5, 713-720.

11.  Largaespada,D., Kaehler,D., Weissinger,E., Mischak,H., Mushinski,F., and Risser,R. (1990). The Activity of An Abl-Myc Retrovirus in Fibroblast Cell-Lines and in Lymphocytes. Current Topics in Microbiology and Immunology 166, 91-96.

12.  Mischak,H., Kolch,W., Hofer,F., Weissinger,E., Gessl,A., Davidson,W.F., Aiello,F.B., Blaas,D., and Rapp,U.R. (1990). A Raf/Myc Virus Immortalized Macrophage Cell-Line Which Supports the Growth of B-Cell and B-Cell Hybridomas. Oncogene 5, 1377-1382.

13.  Weissinger,E.M., Largaespada,D., Smithgill,S.J., Risser,R., Mushinski,J.F., and Mischak,H. (1990). A Retrovirus Expressing V-Abl and C-Myc Induces Plasmacytomas in 100-Percent of Adult Pristane-Primed Balb/C Mice. Current Topics in Microbiology and Immunology 166, 121-127.

14.  Mischak,H., Kolch,W., Goodnight,J., Davidson,W.F., Rapp,U., Rosejohn,S., and Mushinski,J.F. (1991). Expression of Protein-Kinase-C Genes in Hematopoietic-Cells Is Cell-Type-Differentiation and B-Cell-Differentiation Stage Specific. Journal of Immunology 147, 3981-3987.

15.  Mischak,H., Bodenteich,A., Kolch,W., Goodnight,J., Hofer,F., and Mushinski,J.F. (1991). Mouse Protein-Kinase C-Delta, the Major Isoform Expressed in Mouse Hematopoietic-Cells - Sequence of the Cdna, Expression Patterns, and Characterization of the Protein. Biochemistry 30, 7925-7931.

16.  Weissinger,E.M., Mischak,H., Largaespada,D.A., Kaehler,D.A., Mitchell,T., Smithgill,S.J., Risser,R., and Mushinski,J.F. (1991). Induction of Plasmacytomas Secreting Antigen-Specific Monoclonal-Antibodies with A Retrovirus Expressing V-Abl and C-Myc. Proceedings of the National Academy of Sciences of the United States of America 88, 8735-8739.

17.  Dlugosz,A.A., Mischak,H., Mushinski,J.F., and Yuspa,S.H. (1992). Transcripts Encoding Protein Kinase-C-Alpha, Kinase-C-Delta, Kinase-C, Kinase-C-Zeta, and Kinase-C-Eta Are Expressed in Basal and Differentiating Mouse Keratinocytes Invitro and Exhibit Quantitative Changes in Neoplastic-Cells. Molecular Carcinogenesis 5, 286-292.

18.  Goodnight,J., Kazanietz,M.G., Blumberg,P.M., Mushinski,J.F., and Mischak,H. (1992). The cDNA sequence, expression pattern and protein characteristics of mouse protein kinase C-zeta. Gene 122, 305-311.

19.  Largaespada, D., Kaehler, D., Mischak, H., Weissinger, E., Poster, D., Mushinsky, J. F., and Risser, R. A retrovirus that express v-abl and c-myc oncogenes rapidly induces plasmacytomas. Oncogene 7[4], 811-819. 1992.
Ref Type: Journal (Full)

20.  Arnold,T.P., Standaert,M.L., Hernandez,H., Watson,J., Mischak,H., Kazanietz,M.G., Zhao,L.M., Cooper,D.R., and Farese,R.V. (1993). Effects of Insulin and Phorbol Esters on Marcks (Myristoylated Alanine-Rich C-Kinase Substrate) Phosphorylation (and Other Parameters of Protein-Kinase-C Activation) in Rat Adipocytes, Rat Soleus Muscle and Bc3H-1 Myocytes. Biochemical Journal 295, 155-164.

21.  Kazanietz,M.G., Areces,L.B., Bahador,A., Mischak,H., Goodnight,J., Mushinski,J.F., and Blumberg,P.M. (1993). Characterization of Ligand and Substrate-Specificity for the Calcium-Dependent and Calcium-Independent Protein-Kinase-C Isozymes. Molecular Pharmacology 44, 298-307.

22.  Kolch,W., Heidecker,G., Kochs,G., Hummel,R., Vahidi,H., Mischak,H., Finkenzeller,G., Marme,D., and Rapp,U.R. (1993). Protein Kinase-C-Alpha Activates Raf-1 by Direct Phosphorylation. Nature 364, 249-252.

23.  Martiny-Baron,G., Kazanietz,M.G., Mischak,H., Blumberg,P.M., Kochs,G., Hug,H., Marme,D., and Schachtele,C. (1993). Selective inhibition of protein kinase C isozymes by the indolocarbazole Go 6976. J Biol. Chem. 268, 9194-9197.

24.  Mischak,H., Pierce,J.H., Goodnight,J., Kazanietz,M.G., Blumberg,P.M., and Mushinski,J.F. (1993). Phorbol Ester-Induced Myeloid Differentiation Is Mediated by Protein Kinase-C-Alpha and Kinase-C-Delta and Not by Protein Kinase-C-Beta-Ii, Kinase-C-Delta, Kinase-C-Zeta, and Kinase-C-Eta. Journal of Biological Chemistry 268, 20110-20115.

25.  Mischak,H., Goodnight,J., Henderson,D.W., Osada,S., Ohno,S., and Mushinski,J.F. (1993). Unique Expression Pattern of Protein-Kinase-C -Theta - High Messenger-Rna Levels in Normal Mouse Testes and in T-Lymphocytic Cells and Neoplasms. Febs Letters 326, 51-55.

26.  Mischak,H., Goodnight,J., Kolch,W., Martinybaron,G., Schaechtle,C., Kazanietz,M.G., Blumberg,P.M., Pierce,J.H., and Mushinski,J.F. (1993). Overexpression of Protein Kinase-C-Delta and Kinase-Epsilon in Nih 3T3 Cells Induces Opposite Effects on Growth, Morphology, Anchorage Dependence, and Tumorigenicity. Journal of Biological Chemistry 268, 6090-6096.

27.  Ozawa,K., Szallasi,Z., Kazanietz,M.G., Blumberg,P.M., Mischak,H., Mushinski,J.F., and Beaven,M.A. (1993). Ca(2+)-dependent and Ca(2+)-independent isozymes of protein kinase C mediate exocytosis in antigen-stimulated rat basophilic RBL-2H3 cells. Reconstitution of secretory responses with Ca2+ and purified isozymes in washed permeabilized cells. J Biol. Chem. 268 , 1749-1756.

28.  Weissinger,E.M., Mischak,H., Goodnight,J., Davidson,W.F., and Mushinski,J.F. (1993). Addition of Constitutive C-Myc Expression to Abelson Murine Leukemia-Virus Changes the Phenotype of the Cells Transformed by the Virus from Pre-B-Cell Lymphomas to Plasmacytomas. Molecular and Cellular Biology 13, 2578-2585.

29.  Eissner,G., Kolch,W., Mischak,H., Bornkamm,G.W., and Holler,E. (1994). Differential Role of Protein-Kinase-C in Cytokine-Induced Lymphocyte-Endothelium Interaction In-Vitro. Scandinavian Journal of Immunology 40, 395-402.

30.  Farese,R.V., Standaert,M.L., Arnold,T.P., Yamada,K., Musunuru,K., Hernandez,H., Mischak,H., and Cooper,D.R. (1994). Preferential Activation of Microsomal Diacylglycerol/Protein Kinase-C Signaling During Glucose Treatment (De-Novo Phospholipid-Synthesis) of Rat Adipocytes. Journal of Clinical Investigation 93, 1894-1899.

31.  Goodnight,J., Mischak,H., and Mushinski,J.F. (1994). Association of Elevated Levels of Protein-Kinase C-Zeta Messenger-Rna and Protein with Murine B-Lymphocytic Neoplasia. Molecular Carcinogenesis 11, 131-137.

32.  Goodnight,J., Mischak,H., and Mushinski,J.F. (1994). Selective Involvement of Protein-Kinase-C Isozymes in Differentiation and Neoplastic Transformation. Advances in Cancer Research, Vol 64 64, 159-209.

33.  Hafner,S., Adler,H.S., Mischak,H., Janosch,P., Heidecker,G., Wolfman,A., Pippig,S., Lohse,M., Ueffing,M., and Kolch,W. (1994). Mechanism of Inhibition of Raf-1 by Protein-Kinase-A. Molecular and Cellular Biology 14, 6696-6703.

34.  Huppi,K., Siwarski,D., Goodnight,J., and Mischak,H. (1994). Assignment of the Protein-Kinase-C-Delta Polypeptide Gene (Prkcd) to Human-Chromosome-3 and Mouse Chromosome-14. Genomics 19, 161-162.

35.  Kazanietz,M.G., Bustelo,X.R., Barbacid,M., Kolch,W., Mischak,H., Wong,G., Pettit,G.R., Bruns,J.D., and Blumberg,P.M. (1994). Zinc-Finger Domains and Phorbol Ester Pharmacophore - Analysis of Binding to Mutated Form of Protein Kinase-C-Tau and the Vav and C-Raf Protooncogene Products. Journal of Biological Chemistry 269, 11590-11594.

36.  Lehel,C., Olah,Z., Mischak,H., Mushinski,J.F., and Anderson,W.B. (1994). Overexpressed Protein-Kinase C-Delta and C-Epsilon Subtypes in Nih 3T3 Cells Exhibit Differential Subcellular-Localization and Differential Regulation of Sodium-Dependent Phosphate-Uptake. Journal of Biological Chemistry 269, 4761-4766.

37.  Li,W.Q., Mischak,H., Yu,J.C., Wang,L.M., Mushinski,J.F., Heidaran,M.A., and Pierce,J.H. (1994). Tyrosine Phosphorylation of Protein-Kinase C-Delta in Response to Its Activation. Journal of Biological Chemistry 269, 2349-2352.

38.  Morawetz,R., Mischak,H., Goodnight,J., Lendenfeld,T., Mushinsky,J.F., and Kubicek,C.P. (1994). A Protein Kinase-Encoding Gene, Pkt1, from Trichoderma-Reesei, Homologous to the Yeast Ypk1 and Ypk2 (Ykr2) Genes. Gene 146, 309-310.

39.  Weissinger,E.M., Henderson,D.W., Mischak,H., Goodnight,J., and Mushinski,J.F. (1994). Induction of Plasmacytomas That Secrete Monoclonal Antipeptide Antibodies by Retroviral Transformation. Journal of Immunological Methods 168, 123-130.

40.  Yamada,K., Standaert,M.L., Yu,B., Mischak,H., Cooper,D.R., and Farese,R.V. (1994). Insulin-Like Effects of Sodium Orthovanadate on Diacylglycerol-Protein Kinase-C Signaling in Bc3H-1 Myocytes. Archives of Biochemistry and Biophysics 312, 167-172.

41.  Avignon,A., Standaert,M.L., Yamada,K., Mischak,H., Spencer,B., and Farese,R.V. (1995). Insulin Increases Messenger-Rna Levels of Protein-Kinase-C-Alpha and Protein-Kinase-C-Beta in Rat Adipocytes and Protein-Kinase-C-Alpha, Protein-Kinase-C-Beta and Protein-Kinase-C-Theta in Rat Skeletal-Muscle. Biochemical Journal 308, 181-187.

42.  Avignon,A., Standaert,M.L., Yamada,K., Mischak,H., Spencer,B., and Farese,R.V. (1995). Insulin increases mRNA levels of protein kinase C-alpha and -beta in rat adipocytes and protein kinase C-alpha, -beta and -theta in rat skeletal muscle. Biochem. J 308 ( Pt 1), 181-187.

43.  Chalfant,C.E., Mischak,H., Watson,J.E., Winkler,B.C., Goodnight,J., Farese,R.V., and Cooper,D.R. (1995). Regulation of Alternative Splicing of Protein-Kinase C-Beta by Insulin. Journal of Biological Chemistry 270, 13326-13332.

44.  Crespo,P., Mischak,H., and Gutkind,J.S. (1995). Overexpression of Mammalian Protein-Kinase C-Zeta Does Not Affect the Growth-Characteristics of Nih 3T3 Cells. Biochemical and Biophysical Research Communications 213, 266-272.

45.  Goodnight,J.A., Mischak,H., Kolch,W., and Mushinski,J.F. (1995). Immunocytochemical localization of eight protein kinase C isozymes overexpressed in NIH 3T3 fibroblasts. Isoform-specific association with microfilaments, Golgi, endoplasmic reticulum, and nuclear and cell membranes. J Biol. Chem.  270, 9991-10001.

46.  Kieser,A., Goodnight,J., Kolch,W., Mischak,H., and Mushinski,J.F. (1995). Identification of the Primary Growth-Response Gene, St2/T1, As A Gene Whose Expression Is Differentially Regulated by Different Protein-Kinase-C Isozymes. Febs Letters 372, 189-193.

47.  Li,S.F., Janosch,P., Tanji,M., Rosenfeld,G.C., Waymire,J.C., Mischak,H., Kolch,W., and Sedivy,J.M. (1995). Regulation of Raf-1 Kinase-Activity by the 14-3-3-Family of Proteins. Embo Journal 14, 685-696.

48.  Janosch,P., Schellerer,M., Seitz,T., Reim,P., Eulitz,M., Brielmeier,M., Kolch,W., Sedivy,J.M., and Mischak,H. (1996). Characterization of I kappa B kinases - I kappa B-alpha is not phosphorylated by Raf-1 or protein kinase C isozymes, but is a casein kinase II substrate. Journal of Biological Chemistry 271, 13868-13874.

49.  Kieser,A., Seitz,T., Adler,H.S., Coffer,P., Kremmer,E., Crespo,P., Gutkind,J.S., Henderson,D.W., Mushinski,J.F., Kolch,W., and Mischak,H. (1996). Protein kinase C-zeta reverts v-raf transformation of NIH-3T3 cells. Genes & Development 10, 1455-1466.

50.  Kolch,W., Philipp,A., Mischak,H., Dutil,E.M., Mullen,T.M., Feramisco,J.R., Meinkoth,J.L., and Rose,D.W. (1996). Inhibition of Raf-1 signaling by a monoclonal antibody, which interferes with Raf-1 activation and with Mek substrate binding. Oncogene 13, 1305-1314.

51.  Mischak,H., Seitz,T., Janosch,P., Eulitz,M., Steen,H., Schellerer,M., Philipp,A., and Kolch,W. (1996). Negative regulation of Raf-1 by phosphorylation of serine 621. Molecular and Cellular Biology 16, 5409-5418.

52.  Morawetz,R., Lendenfeld,T., Mischak,H., Muhlbauer,M., Gruber,F., Goodnight,J., deGraaff,L.H., Visser,J., Mushinski,J.F., and Kubicek,C.P. (1996). Cloning and characterisation of genes (pkc1 and pkcA) encoding protein kinase C homologues from Trichoderma reesei and Aspergillus niger. Molecular & General Genetics 250, 17-28.

53.  Perletti,G.P., Folini,M., Lin,H.C., Mischak,H., Piccinini,F., and Tashjian,A.H. (1996). Overexpression of protein kinase C epsilon is oncogenic in rat colonic epithelial cells. Oncogene 12, 847-854.

54.  Bossenmaier,B., Mosthaf,L., Mischak,H., Ullrich,A., and Haring,H.U. (1997). Protein kinase C isoforms beta 1 and beta 2 inhibit the tyrosine kinase activity of the insulin receptor. Diabetologia 40, 863-866.

55.  Kellerer,M., Mushack,J., Mischak,H., and Haring,H.U. (1997). Protein kinase C (PKC) epsilon enhances the inhibitory effect of TNF alpha on insulin signaling in HEK293 cells. Febs Letters 418, 119-122.

56.  Ueffing,M., Lovric,J., Philipp,A., Mischak,H., and Kolch,W. (1997). Protein kinase C-epsilon associates with the Raf-1 kinase and induces the production of growth factors that stimulate Raf-1 activity. Oncogene 15, 2921-2927.

57.  Wang,Q.J., Acs,P., Goodnight,J., Giese,T., Blumberg,P.M., Mischak,H., and Mushinski,J.F. (1997). The catalytic domain of protein kinase C-delta in reciprocal delta and epsilon chimeras mediates phorbol ester-induced macrophage differentiation of mouse promyelocytes. J Biol. Chem. 272 , 76-82.

58.  Weissinger,E.M., Eissner,G., Grammer,C., Fackler,S., Haefner,B., Yoon,L.S., Lu,K.S., Bazarov,A., Sedivy,J.M., Mischak,H., and Kolch,W. (1997). Inhibition of the Raf-1 kinase by cyclic AMP agonists causes apoptosis of v-abl-transformed cells. Molecular and Cellular Biology 17, 3229-3241.

59.  Baumann,M., Mischak,H., Dammeier,S., Kolch,W., Gires,O., Pich,D., Zeidler,R., Delecluse,H.J., and Hammerschmidt,W. (1998). Activation of the Epstein-Barr virus transcription factor BZLF1 by 12-O-tetradecanoylphorbol-13-acetate-induced phosphorylation. Journal of Virology 72, 8105-8114.

60.  Hacker,H., Mischak,H., Miethke,T., Liptay,S., Schmid,R., Sparwasser,T., Heeg,K., Lipford,G.B., and Wagner,H. (1998). CpG-DNA-specific activation of antigen-presenting cells requires stress kinase activity and is preceded by non-specific endocytosis and endosomal maturation. Embo Journal 17, 6230-6240.

61.  Kellerer,M., Mushack,J., Seffer,E., Mischak,H., Ullrich,A., and Haring,H.U. (1998). Protein kinase C isoforms alpha, delta and theta require insulin receptor substrate-1 to inhibit the tyrosine kinase activity of the insulin receptor in human kidney embryonic cells (HEK 293 cells). Diabetologia  41, 833-838.

62.  Lovric,J., Dammeier,S., Kieser,A., Mischak,H., and Kolch,W. (1998). Activated Raf induces the hyperphosphorylation of stathmin and the reorganization of the microtubule network. Journal of Biological Chemistry 273, 22848-22855.

63.  Maucher,C., Weissinger,E.M., Kremmer,E., Baccarini,M., Procyk,K., Henderson,D.W., Wolff,L., Kolch,W., Kaspers,B., Mushinski,J.F., and Mischak,H. (1998). Activation of bcl-2 suppressible 40 and 44 kDa p38-like kinases during apoptosis of early and late B lymphocytic cell lines. Febs Letters 427, 29-35.

64.  Muller,G., Storz,P., Bourteele,S., Doppler,H., Pfizenmaier,K., Mischak,H., Philipp,A., Kaiser,C., and Kolch,W. (1998). Regulation of Raf-1 kinase by TNF via its second messenger ceramide and cross-talk with mitogenic signalling. Embo Journal 17, 732-742.

65.  Prouty,S.M., Maroo,A., Maucher,C., Mischak,H., Kolch,W., and Sedivy,J.M. (1998). Studies of perinuclear and nuclear translocation of the Raf-1 protein in rodent fibroblasts. Biochimica et Biophysica Acta-Molecular Cell Research 1402, 6-16.

66.  Wang,Q.J., Acs,P., Goodnight,J.A., Blumberg,P.M., Mischak,H., and Mushinski,J.F. (1998). The catalytic domain of PKC-epsilon, in reciprocal PKC-delta and -epsilon chimeras, is responsible for conferring tumorgenicity to NIH3T3 cells, whereas both regulatory and catalytic domains of PKC-epsilon contribute to in vitro transformation. Oncogene 16, 53-60.

67.  Wooten,M.W., Seibenhener,M.L., Heikkila,J.E., and Mischak,H. (1998). delta-protein kinase C phosphorylation parallels inhibition of nerve growth factor-induced differentiation independent of changes in Trk A and MAP kinase signalling in PC12 cells. Cellular Signalling 10, 265-276.

68.  Caloca,M.J., Garcia-Bermejo,M.L., Blumberg,P.M., Lewin,N.E., Kremmer,E., Mischak,H., Wang,S.M., Nacro,K., Bienfait,B., Marquez,V.E., and Kazanietz,M.G. (1999). beta 2-chimaerin is a novel target for diacylglycerol: Binding properties and changes in subcellular localization mediated by ligand binding to its C1 domain. Proceedings of the National Academy of Sciences of the United States of America 96, 11854-11859.

69.  Cooper,D.R., Watson,J.E., Patel,N., Illingworth,P., cevedo-Duncan,M., Goodnight,J., Chalfant,C.E., and Mischak,H. (1999). Ectopic expression of protein kinase C beta II, -delta, and -epsilon, but not -beta I or -zeta, provide for insulin stimulation of glucose uptake in NIH-3T3 cells. Archives of Biochemistry and Biophysics 372, 69-79.

70.  Hacker,H., Mischak,H., Hacker,G., Eser,S., Prenzel,N., Ullrich,A., and Wagner,H. (1999). Cell type-specific activation of mitogen-activated protein kinases by CpG-DNA controls interleukin-12 release from antigen-presenting cells. Embo Journal 18, 6973-6982.

71.  Mai,S., Hanley-Hyde,J., Rainey,G.J., Kuschak,T.I., Paul,J.T., Littlewood,T.D., Mischak,H., Stevens,L.M., Henderson,D.W., and Mushinski,J.F. (1999). Chromosomal and extrachromosomal instability of the cyclin D2 gene is induced by Myc overexpression. Neoplasia. 1, 241-252.

72.  Yeung,K., Seitz,T., Li,S.F., Janosch,P., McFerran,B., Kaiser,C., Fee,F., Katsanakis,K.D., Rose,D.W., Mischak,H., Sedivy,J.M., and Kolch,W. (1999). Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature 401, 173-177.

73.  Abraham,D., Podar,K., Pacher,M., Kubicek,M., Welzel,N., Hemmings,B.A., Dilworth,S.M., Mischak,H., Kolch,W., and Baccarini,M. (2000). Raf-1-associated protein phosphatase 2A as a positive regulator of kinase activation. Journal of Biological Chemistry 275, 22300-22304.

74.  Baumann,M., Gires,O., Kolch,W., Mischak,H., Zeidler,R., Pich,D., and Hammerschmidt,W. (2000). The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1. European Journal of Biochemistry 267, 3891-3901.

75.  Dammeier,S., Lovric,J., Eulitz,M., Kolch,W., Mushinski,J.F., and Mischak,H. (2000). Identification of the smooth muscle-specific protein, sm22, as a novel protein kinase C substrate using two-dimensional gel electrophoresis and mass spectrometry. Electrophoresis 21, 2443-2453.

76.  Janosch,P., Kieser,A., Eulitz,M., Lovric,J., Sauer,G., Reichert,M., Gounari,F., Buscher,D., Baccarini,M., Mischak,H., and Kolch,W. (2000). The Raf-1 kinase associates with vimentin kinases and regulates the structure of vimentin filaments. Faseb Journal 14, 2008-2021.

77.  Yeung,K., Janosch,P., McFerran,B., Rose,D.W., Mischak,H., Sedivy,J.M., and Kolch,W. (2000). Mechanism of suppression of the Raf/MEK/extracellular signal-regulated kinase pathway by the Raf kinase inhibitor protein. Molecular and Cellular Biology 20, 3079-3085.

78.  Krasel,C., Dammeier,S., Winstel,R., Brockmann,J., Mischak,H., and Lohse,M.J. (2001). Phosphorylation of GRK2 by protein kinase C abolishes its inhibition by calmodulin. Journal of Biological Chemistry 276 , 1911-1915.

79.  Brandt,D., Gimona,M., Hillmann,M., Haller,H., and Mischak,H. (2002). Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway. Journal of Biological Chemistry 277, 20903-20910.

80.  Dhillon,A.S., Pollock,C., Steen,H., Shaw,P.E., Mischak,H., and Kolch,W. (2002). Cyclic AMP-dependent kinase regulates Raf-1 kinase mainly by phosphorylation of serine 259. Molecular and Cellular Biology 22, 3237-3246.

81.  Goerke,A., Sakai,N., Gutjahr,E., Schlapkohl,W.A., Mushinski,J.F., Haller,H., Kolch,W., Saito,N., and Mischak,H. (2002). Induction of apoptosis by protein kinase C delta is independent of its kinase activity. Journal of Biological Chemistry 277, 32054-32062.

82.  Hennige,A.M., Fritsche,A., Strack,V., Weigert,C., Mischak,H., Borboni,P., Renn,W., Haring,H.U., and Kellerer,M. (2002). PKC zeta enhances insulin-like growth factor 1-dependent mitogenic activity in the rat clonal beta cell line RIN 1046-38. Biochemical and Biophysical Research Communications 290, 85-90.

83.  Walter,R., Mischak,H., and Haller,H. (2002). Haemodialysis, atherosclerosis and inflammation - identifying molecular mechanisms of chronic vascular disease in ESDR patients. Nephrology Dialysis Transplantation 17, 24-29.

84.  Brandt,D.T., Goerke,A., Heuer,M., Gimona,M., Leitges,M., Kremmer,E., Lammers,R., Haller,H., and Mischak,H. (2003). Protein kinase C delta induces Src kinase activity via activation of the protein tyrosine phosphatase PTP alpha. Journal of Biological Chemistry 278, 34073-34078.

85.  Chalmers,M.J., Quinn,J.P., Blakney,G.T., Emmett,M.R., Mischak,H., Gaskell,S.J., and Marshall,A.G. (2003). Liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometric characterization of protein kinase C phosphorylation. Journal of Proteome Research 2, 373-382.

86.  Dhillon,A.S., Meikle,S., Peyssonnaux,C., Grindlay,J., Kaiser,C., Steen,H., Shaw,P.E., Mischak,H., Eychene,A., and Kolch,W. (2003). A Raf-1 mutant that dissociates MEK/extracellular signal-regulated kinase activation from malignant transformation and differentiation but not proliferation. Molecular and Cellular Biology 23, 1983-1993.

87.  Eitel,K., Staiger,H., Rieger,J., Mischak,H., Brandhorst,H., Brendel,M.A., Bretzel,R.G., Haring,H.U., and Kellerer,M. (2003). Protein kinase C delta activation and translocation to the nucleus are required for fatty acid-induced apoptosis of insulin-secreting cells. Diabetes 52, 991-997.

88.  Kaiser,T., Hermann,A., Kielstein,J.T., Wittke,S., Bartel,S., Krebs,R., Hausadel,F., Hillmann,M., Golovko,I., Koester,P., Haller,H., Weissinger,E.M., Fliser,D., and Mischak,H. (2003). Capillary electrophoresis coupled to mass spectrometry to establish polypeptide patterns in dialysis fluids. Journal of Chromatography A 1013, 157-171.

89.  Wittke,S., Fliser,D., Haubitz,M., Bartel,S., Krebs,R., Hausadel,F., Hillmann,M., Golovko,I., Koester,P., Haller,H., Kaiser,T., Mischak,H., and Weissinger,E.M. (2003). Determination of peptides and proteins in human urine with capillary electrophoresis-mass spectrometry, a suitable tool for the establishment of new diagnostic markers. Journal of Chromatography A 1013, 173-181.

90.  Canagarajah,B., Leskow,F.C., Ho,J.Y.S., Mischak,H., Saidi,L.F., Kazanietz,M.G., and Hurley,J.H. (2004). Structural mechanism for lipid activation of the Rac-specific GAP, beta 2-chimaerin. Cell 119, 407-418.

91.  Chalmers,M.J., Kolch,W., Emmett,M.R., Marshall,A.G., and Mischak,H. (2004). Identification and analysis of phosphopeptides. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 803, 111-120.

92.  Kaiser,T., Wittke,S., Just,I., Krebs,R., Bartel,S., Fliser,D., Mischak,H., and Weissinger,E.M. (2004). Capillary electrophoresis coupled to mass spectrometer for automated and robust pofypaptide determination in body fluids for clinical use. Electrophoresis 25, 2044-2055.

93.  Kaiser,T., Kamal,H., Rank,A., Kolb,H.J., Holler,E., Ganser,A., Hertenstein,B., Mischak,H., and Weissinger,E.M. (2004). Proteomics applied to the clinical follow-up of patients after allogeneic hematopoietic stem cell transplantation. Blood 104, 340-349.

94.  Kolch,W., Mischak,H., Chalmers,M.J., Pitt,A., and Marshall,A.G. (2004). Clinical proteomics: a question of technology. Rapid Commun. Mass Spectrom. 18, 2365-2366.

95.  Mischak,H., Kaiser,T., Walden,M., Hillmann,M., Wittke,S., Herrmann,A., Knueppel,S., Haller,H., and Fliser,D. (2004). Proteomic analysis for the assessment of diabetic renal damage in humans. Clinical Science 107, 485-495.

96.  Neuhoff,N., Kaiser,T., Wittke,S., Krebs,R., Pitt,A., Burchard,A., Sundmacher,A., Schlegelberger,B., Kolch,W., and Mischak,H. (2004). Mass spectrometry for the detection of differentially expressed proteins: a comparison of surface-enhanced laser desorption/ionization and capillary electrophoresis/mass spectrometry. Rapid Commun. Mass Spectrom. 18, 149-156.

97.  Schaefer,M., Mischak,H., Schnell,S., Griese,A., Iakubov,R., Riepenhausen,G., and Schofl,C. (2004). Mechanisms of arginine-vasopressin-induced Ca2+ oscillations in beta-cells (HIT-T15): A role for oscillating protein kinase C. Endocrinology 145, 4635-4644.

98.  Wang,Q.J., Lu,G., Schlapkohl,W.A., Goerke,A., Larsson,C., Mischak,H., Blumberg,P.M., and Mushinski,J.F. (2004). The V5 domain of protein kinase C plays a critical role in determining the isoform-specific localization, translocation, and biological function of protein kinase C-delta and -epsilon. Mol Cancer Res. 2, 129-140.

99.  Weissinger, E. M. and Mischak, H. Proteom analysis applied towards early diagnosis of renal diseases and transplant-monitoring. Transplantationsmedizin 16, 2-9. 2004.
Ref Type: Journal (Full)

100.     Weissinger,E.M., Oettrich,K., Evans,C., Genieser,H.G., Schwede,F., Dangers,M., Dammann,E., Kolb,H.J., Mischak,H., Ganser,A., and Kolch,W. (2004). Activation of protein kinase A (PKA) by 8-Cl-cAMP as a novel approach for antileukaemic therapy. British Journal of Cancer 91, 186-192.

101.   Weissinger,E.M., Wittke,S., Kaiser,T., Haller,H., Bartel,S., Krebs,R., Golovko,I., Rupprecht,H.D., Haubitz,M., Hecker,H., Mischak,H., and Fliser,D. (2004). Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes. Kidney International 65, 2426-2434.

102.   Weissinger,E.M., Kaiser,T., Meert,N., De Smet,R., Walden,M., Mischak,H., and Vanholder,R.C. (2004). Proteomics: a novel tool to unravel the patho-physiology of uraemia. Nephrology Dialysis Transplantation 19, 3068-3077.

103.   Wittke,S., Kaiser,T., and Mischak,H. (2004). Differential polypeptide display: the search for the elusive target. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 803, 17-26.

104.   Chalmers,M.J., Mackay,C.L., Hendrickson,C.L., Wittke,S., Walden,M., Mischak,H., Fliser,D., Just,I., and Marshall,A.G. (2005). Combined top-down and bottom-up mass spectrometric approach to characterization of biomarkers for renal disease. Analytical Chemistry 77, 7163-7171.

105.   Fliser,D., Wittke,S., and Mischak,H. (2005). Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes. Electrophoresis 26, 2708-2716.

106.   Haubitz,M., Wittke,S., Weissinger,E.M., Walden,M., Rupprecht,H.D., Floege,J., Haller,H., and Mischak,H. (2005). Urine protein patterns can serve as diagnostic tools in patients with IgA nephropathy. Kidney International 67, 2313-2320.

107.   Kolch,W., Mischak,H., and Pitt,A.R. (2005). The molecular make-up of a tumour: proteomics in cancer research. Clinical Science 108, 369-383.

108.   Kolch,W., Neususs,C., Peizing,M., and Mischak,H. (2005). Capillary electrophoresis - Mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery. Mass Spectrometry Reviews 24, 959-977.

109.   Meier,M., Kaiser,T., Herrmann,A., Knueppel,S., Hillmann,M., Koester,P., Danne,T., Haller,H., Fliser,D., and Mischak,H. (2005). Identification of urinary protein pattern in Type 1 diabetic adolescents with early diabetic nephropathy by a novel combined proteome analysis. Journal of Diabetes and Its Complications 19, 223-232.

110.   Rogers,S., Girolami,M., Krebs,R., and Mischak,H. (2005). Disease classification from capillary electrophoresis: Mass spectrometry. Pattern Recognition and Data Mining, Pt 1, Proceedings 3686, 183-191.

111.   Rossing,K., Mischak,H., Parving,H.H., Christensen,P.K., Walden,M., Hillmann,M., and Kaiser,T. (2005). Impact of diabetic nephropathy and angiotensin II receptor blockade on urinary polypeptide patterns. Kidney International 68, 193-205.

112.   Theodorescu,D., Fliser,D., Wittke,S., Mischak,H., Krebs,R., Walden,M., Ross,M., Eltze,E., Bettendorf,O., Wulfing,C., and Semjonow,A. (2005). Pilot study of capillary electrophoresis coupled to mass spectrometry as a tool to define potential prostate cancer biomarkers in urine. Electrophoresis 26, 2797-2808.

113.   Weissinger,E.M., Hertenstein,B., Mischak,H., and Ganser,A. (2005). Online coupling of capillary electrophoresis with mass spectrometry for the identification of biomarkers for clinical diagnosis. Expert Review of Proteomics 2, 639-647.

114.   Wittke,S., Haubitz,M., Walden,M., Rohde,F., Schwarz,A., Mengel,M., Mischak,H., Haller,H., and Gwinner,W. (2005). Detection of acute tubulointerstitial rejection by proteomic analysis of urinary samples in renal transplant recipients. American Journal of Transplantation 5, 2479-2488.

115.   Wittke,S., Mischak,H., Walden,M., Kolch,W., Radler,T., and Wiedemann,K. (2005). Discovery of biomarkers in human urine and cerebrospinal fluid by capillary electrophoresis coupled to mass spectrometry: Towards new diagnostic and therapeutic approaches. Electrophoresis 26, 1476-1487.

116.   Decramer,S., Wittke,S., Mischak,H., Zurbig,P., Walden,M., Bouissou,F., Bascands,J.L., and Schanstra,J.P. (2006). Predicting the clinical outcome of congenital unilateral ureteropelvic junction obstruction in newborn by urinary proteome analysis. Nature Medicine 12, 398-400.

117.   Girolami, M., Mischak, H., and Krebs, R. Analysis of Complex, Multi-Dimensional Data Sets. Drug Discovery Today 3, 13-19. 2006.
Ref Type: Journal (Full)

118.   Schiffer,E., Mischak,H., and Novak,J. (2006). High resolution proteome/peptidome analysis of body fluids by capillary electrophoresis coupled with MS. Proteomics 6, 5615-5627.

119.   Theodorescu,D., Wittke,S., Ross,M.M., Walden,M., Conaway,M., Just,I., Mischak,H., and Frierson,H.F. (2006). Discovery and validation of new protein biomarkers for 4 urothelial cancer: a prospective analysis. Lancet Oncology 7, 230-240.

120.   Weissinger,E.M., Mischak,H., Ganser,A., and Hertenstein,B. (2006). Value of proteomics applied to the follow-up in stem cell transplantation. Annals of Hematology 85, 205-211.

121.   Weissinger,E.M., Nguyen-Khoa,T., Fumeron,C., Saltiel,C., Walden,M., Kaiser,T., Mischak,H., Drueke,T.B., Lacour,B., and Massy,Z.A. (2006). Effects of oral vitamin C supplementation in hemodialysis patients: A proteomic assessment. Proteomics 6, 993-1000.

122.   Zurbig,P., Renfrow,M.B., Schiffer,E., Novak,J., Walden,M., Wittke,S., Just,I., Pelzing,M., Neususs,C., Theodorescu,D., Root,K.E., Ross,M.M., and Mischak,H. (2006). Biomarker discovery by CE-MS enables sequence analysis via MS/MS with platform-independent separation. Electrophoresis 27, 2111-2125.

123.   Fliser,D., Novak,J., Thongboonkerd,V., Argiles,A., Jankowski,V., Girolami,M.A., Jankowski,J., and Mischak,H. (2007). Advances in urinary proteome analysis and biomarker discovery. Journal of the American Society of Nephrology 18, 1057-1071.

124.   Frommberger,M., Zurbig,P., Jantos,J., Krahn,T., Mischak,H., Pich,A., Just,I., Schmitt-Kopplin,P., and Schiffer,E. (2007). Peptidomic analysis of rat urine using capillary electrophoresis coupled to mass spectrometry. Proteomics Clinical Applications 1, 650-660.

125.   Good,D.M., Thongboonkerd,V., Novak,J., Bascands,J.L., Schanstra,J.P., Coon,J.J., Dominiczak,A., and Mischak,H. (2007). Body fluid proteomics for biomarker discovery: Lessons from the past hold the key to success in the future. Journal of Proteome Research 6, 4549-4555.

126.   Goodsaid,F., Bandow,J.E., and Mischak,H. (2007). Grand rounds in proteomics at the FDA - White Oak, Silver Spring, MD, USA, April 3, 2007. Proteomics Clinical Applications 1, 1526-1531.

127.   Julian,B.A., Wittke,S., Novak,J., Good,D.M., Coon,J.J., Kellmann,M., Zurbig,P., Schiffer,E., Haubitz,M., Moldoveanu,Z., Calcatera,S.M., Wyatt,R.J., Sykora,J., Sladkova,E., Hes,O., Mischak,H., and McGuire,B.M. (2007). Electrophoretic methods for analysis of urinary polypeptides in IgA-associated renal diseases. Electrophoresis 28, 4469-4483.

128.   Mischak,H., Julian,B.A., and Novak,J. (2007). High-resolution proteome/peptidome analysis of peptides and low-molecular-weight proteins in urine. Proteomics Clinical Applications 1, 792-804.

129.   Mischak,H., Apweiler,R., Banks,R.E., Conaway,M., Coon,J., Dominiczak,A., Ehrich,J.H.H., Fliser,D., Girolami,M., Hermjakob,H., Hochstrasser,D., Jankowski,J., Julian,B.A., Kolch,W., Massy,Z.A., Neusuess,C., Novak,J., Peter,K., Rossing,K., Schanstra,J., Semmes,O.J., Theodorescu,D., Thongboonkerd,V., Weissinger,E.M., Van Eyk,J.E., and Yamamoto,T. (2007). Clinical proteomics: A need to define the field and to begin to set adequate standards. Proteomics Clinical Applications 1, 148-156.

130.   Sniehotta,M., Schiffer,E., Zurbig,P., Novak,J., and Mischak,H. (2007). CE - a multifunctional application for clinical diagnosis. Electrophoresis 28, 1407-1417.

131.   Theodorescu,D. and Mischak,H. (2007). Mass spectrometry based proteomics in urine biomarker discovery. World Journal of Urology 25, 435-443.

132.   Vanholder,R., Meert,N., Schepers,E., Glorieux,G., Argiles,A., Brunet,P., Cohen,G., Drueke,T., Mischak,H., Spasovski,G., Massy,Z., and Jankowski,J. (2007). Review on uraemic solutes II - Variability in reported concentrations: causes and consequences. Nephrology Dialysis Transplantation 22, 3115-3121.

133.   Weissinger,E.M. and Mischak,H. (2007). Application of proteomics to posttransplantational follow-up. Methods Mol Med. 134, 217-228.

134.   Coon,J.J., Zurbig,P., Dakna,M., Dominicza,A.F., Decramer,S., Fliser,D., Frommberger,M., Golovko,I., Good,D.M., Herget-Rosenthal,S., Jankowski,J., Julian,B.A., Kellmann,M., Kolch,W., Massy,Z., Novak,J., Rossing,K., Schanstra,J.P., Schiffer,E., Theodorescu,D., Vanholder,R., Weissinger,E.M., Mischak,H., and Schmitt-Kopplin,P. (2008). CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics. Proteomics Clinical Applications 2, 964-973.

135.   Decramer,S., de Peredo,A.G., Breuil,B., Mischak,H., Monsarrat,B., Bascands,J.L., and Schanstra,J.P. (2008). Urine in Clinical Proteomics. Molecular & Cellular Proteomics 7, 1850-1862.

136.   Jiang,H., Schiffer,E., Song,Z.F., Wang,J.W., Zurbig,P., Thedieck,K., Moes,S., Bantel,H., Saal,N., Jantos,J., Brecht,M., Jeno,P., Hall,M.N., Hager,K., Manns,M.P., Hecker,H., Ganser,A., Dohner,K., Bartke,A., Meissner,C., Mischak,H., Ju,Z.Y., and Rudolph,K.L. (2008). Proteins induced by telomere dysfunction and DNA damage represent biomarkers of human aging and disease. Proceedings of the National Academy of Sciences of the United States of America 105, 11299-11304.

137.   Mathivanan,S., Ahmed,M., Ahn,N.G., Alexandre,H., Amanchy,R., Andrews,P.C., Bader,J.S., Balgley,B.M., Bantscheff,M., Bennett,K.L., Bjorling,E., Blagoev,B., Bose,R., Brahmachari,S.K., Burlingame,A.S., Bustelo,X.R., Cagney,G., Cantin,G.T., Cardasis,H.L., Celis,J.E., Chaerkady,R., Chu,F., Cole,P.A., Costello,C.E., Cotter,R.J., Crockett,D., DeLany,J.P., De Marzo,A.M., DeSouza,L.V., Deutsch,E.W., Dransfield,E., Drewes,G., Droit,A., Dunn,M.J., Elenitoba-Johnson,K., Ewing,R.M., Van,E.J., Faca,V., Falkner,J., Fang,X., Fenselau,C., Figeys,D., Gagne,P., Gelfi,C., Gevaert,K., Gimble,J.M., Gnad,F., Goel,R., Gromov,P., Hanash,S.M., Hancock,W.S., Harsha,H.C., Hart,G., Hays,F., He,F., Hebbar,P., Helsens,K., Hermeking,H., Hide,W., Hjerno,K., Hochstrasser,D.F., Hofmann,O., Horn,D.M., Hruban,R.H., Ibarrola,N., James,P., Jensen,O.N., Jensen,P.H., Jung,P., Kandasamy,K., Kheterpal,I., Kikuno,R.F., Korf,U., Korner,R., Kuster,B., Kwon,M.S., Lee,H.J., Lee,Y.J., Lefevre,M., Lehvaslaiho,M., Lescuyer,P., Levander,F., Lim,M.S., Lobke,C., Loo,J.A., Mann,M., Martens,L., Martinez-Heredia,J., McComb,M., McRedmond,J., Mehrle,A., Menon,R., Miller,C.A., Mischak,H., Mohan,S.S., Mohmood,R., Molina,H., Moran,M.F., Morgan,J.D., Moritz,R., Morzel,M., Muddiman,D.C., Nalli,A., Navarro,J.D., Neubert,T.A., Ohara,O., Oliva,R., Omenn,G.S., Oyama,M., Paik,Y.K., Pennington,K., Pepperkok,R., Periaswamy,B., Petricoin,E.F., Poirier,G.G., Prasad,T.S., Purvine,S.O., Rahiman,B.A., Ramachandran,P., Ramachandra,Y.L., Rice,R.H., Rick,J., Ronnholm,R.H., Salonen,J., Sanchez,J.C., Sayd,T., Seshi,B., Shankari,K., Sheng,S.J., Shetty,V., Shivakumar,K., Simpson,R.J., Sirdeshmukh,R., Siu,K.W., Smith,J.C., Smith,R.D., States,D.J., Sugano,S., Sullivan,M., Superti-Furga,G., Takatalo,M., Thongboonkerd,V., Trinidad,J.C., Uhlen,M., Vandekerckhove,J., Vasilescu,J., Veenstra,T.D., Vidal-Taboada,J.M., Vihinen,M., Wait,R., Wang,X., Wiemann,S., Wu,B., Xu,T., Yates,J.R., Zhong,J., Zhou,M., Zhu,Y., Zurbig,P., and Pandey,A. (2008). Human Proteinpedia enables sharing of human protein data. Nat. Biotechnol. 26, 164-167.

138.   Raedler,T.J., Wittke,S., Jahn,H., Koessler,A., Mischak,H., and Wiedemann,K. (2008). Capillary electrophoresis mass spectrometry as a potential tool to detect lithium-induced nephropathy: Preliminary results. Progress in Neuro-Psychopharmacology & Biological Psychiatry 32, 673-678.

139.   Rossing,K., Mischak,H., Rossing,P., Schanstra,J.P., Wiseman,A., and Maahs,D.M. (2008). The urinary proteome in diabetes and diabetes-associated complications: New ways to assess disease progression and evaluate therapy. Proteomics Clinical Applications 2, 997-1007.

140.   Rossing,K., Mischak,H., Dakna,M., Zurbig,P., Novak,J., Julian,B.A., Good,D.M., Coon,J.J., Tarnow,L., and Rossing,P. (2008). Urinary proteomics in diabetes and CKD. Journal of the American Society of Nephrology 19, 1283-1290.

141.   Schiffer,E., Mischak,H., and Vanholder,R.C. (2008). Exploring the uremic toxins using proteomic technologies. Proteomics in Nephrology - Towards Clinical Applications 160, 159-171.

142.   Schiffer,E., Mischak,H., Theodorescu,D., and Vlahou,A. (2008). Challenges of using mass spectrometry as a bladder cancer biomarker discovery platform. World Journal of Urology 26, 67-74.

143.   Theodorescu,D., Schiffer,E., Bauer,H.W., Douwes,F., Eichhorn,F., Polley,R., Schmidt,T., Schofer,W., Zurbig,P., Good,D.M., Coon,J.J., and Mischak,H. (2008). Discovery and validation of urinary biomarkers for propstate cancer. Proteomics Clinical Applications 2, 556-570.

144.   Vlahou,A., Schanstra,J., Frokiaer,J., El,N.M., Spasovski,G., Mischak,H., Domon,B., Allmaier,G., Bongcam-Rudloff,E., and Attwood,T. (2008). Establishment of a European Network for Urine and Kidney Proteomics. J Proteomics 71, 490-492.

145.   Zimmerli,L.U., Schiffer,E., Zurbig,P., Good,D.M., Kellmann,M., Mouls,L., Pitt,A.R., Coon,J.J., Schmieder,R.E., Peter,K.H., Mischak,H., Kolch,W., Delles,C., and Dominiczak,A.F. (2008). Urinary proteomic biomarkers on coronary artery disease. Molecular & Cellular Proteomics 7, 290-298.

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149.   Haubitz,M., Good,D.M., Woywodt,A., Haller,H., Rupprecht,H., Theodorescu,D., Dakna,M., Coon,J.J., and Mischak,H. (2009). Identification and validation of urinary biomarkers for differential diagnosis and evaluation of therapeutic intervention in anti-neutrophil cytoplasmic antibody-associated vasculitis. Mol Cell Proteomics 8, 2296-2307.

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152.   Kistler,A.D., Mischak,H., Poster,D., Dakna,M., Wuthrich,R.P., and Serra,A.L. (2009). Identification of a unique urinary biomarker profile in patients with autosomal dominant polycystic kidney disease. Kidney Int. 76, 89-96.

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154.   Metzger,J., Luppa,P.B., Good,D.M., and Mischak,H. (2009). Adapting mass spectrometry-based platforms for clinical proteomics applications: The capillary electrophoresis coupled mass spectrometry paradigm. Crit Rev. Clin. Lab Sci. 1-24.

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156.   Mischak,H., Espandiari,P., Sadrieh,N., and Hanig,J. (2009). Profiling of rat urinary proteomic patterns associated with drug-induced nephrotoxicity using CE coupled with MS as a potential model for detection of drug-induced adverse effects. Proteomics Clin Appl. 3, 1062-1071.

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158.   Schiffer,E., Mischak,H., and Zimmerli,L.U. (2009). Proteomics in gerontology: current applications and future aspects--a mini-review. Gerontology 55, 123-137.

159.   Schiffer,E., Vlahou,A., Petrolekas,A., Stravodimos,K., Tauber,R., Geschwend,J.E., Neuhaus,J., Stolzenburg,J.U., Conaway,M.R., Mischak,H., and Theodorescu,D. (2009). Prediction of muscle-invasive bladder cancer using urinary proteomics. Clin. Cancer Res. 15, 4935-4943.

160.   Snell-Bergeon,J.K., Maahs,D.M., Ogden,L.G., Kinney,G.L., Hokanson,J.E., Schiffer,E., Rewers,M., and Mischak,H. (2009). Evaluation of urinary biomarkers for coronary artery disease, diabetes, and diabetic kidney disease. Diabetes Technol. Ther. 11, 1-9.

161.   Vanholder,R., bou-Deif,O., Argiles,A., Baurmeister,U., Beige,J., Brouckaert,P., Brunet,P., Cohen,G., De Deyn,P.P., Drueke,T.B., Fliser,D., Glorieux,G., Herget-Rosenthal,S., Horl,W.H., Jankowski,J., Jorres,A., Massy,Z.A., Mischak,H., Perna,A.F., Rodriguez-Portillo,J.M., Spasovski,G., Stegmayr,B.G., Stenvinkel,P., Thornalley,P.J., Wanner,C., and Wiecek,A. (2009). The role of EUTox in uremic toxin research. Semin. Dial 22, 323-328.

162.   Vlahou,A., Allmaier,G., Attwood,T., Bongcam-Rudloff,E., Charonis,A., Frokiaer,J., Mischak,H., Schanstra,J., and Spasovski,G. (2009). 2nd Combined Working Group and Management Committee Meeting of Urine and Kidney Proteomics COST Action 29-30 March 2009, Nafplio, Greece. Proteomics Clin Appl. 3, 1017-1022.

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164.   Weissinger,E.M., Mischak,H., Kontsendorn,J., Hahn,A., Hahn,N., Morgan,M., and Ganser,A. (2009). Proteome analysis in hematology using capillary electrophoresis coupled on-line to mass spectrometry. Mini. Rev. Med. Chem. 9, 627-3.

165.   Zurbig,P., Decramer,S., Dakna,M., Jantos,J., Good,D.M., Coon,J.J., Bandin,F., Mischak,H., Bascands,J.L., and Schanstra,J.P. (2009). The human urinary proteome reveals high similarity between kidney aging and chronic kidney disease. Proteomics 9, 2108-2117.

166.   Zurbig,P., Schiffer,E., and Mischak,H. (2009). Capillary electrophoresis coupled to mass spectrometry for proteomic profiling of human urine and biomarker discovery. Methods Mol Biol. 564, 105-121.

167.   Alkhalaf,A., Zurbig,P., Bakker,S.J.L., Bilo,H.J.G., Cerna,M., Fischer,C., Fuchs,S., Janssen,B., Medek,K., Mischak,H., Roob,J.M., Rossing,K., Rossing,P., Rychlik,I., Sourij,H., Tiran,B., Winklhofer-Roob,B.M., and Navis,G.J. (2010). Multicentric Validation of Proteomic Biomarkers in Urine Specific for Diabetic Nephropathy. Plos One 5.

168.   Andersen,S., Mischak,H., Zurbig,P., Parving,H.H., and Rossing,P. (2010). Urinary proteome analysis enables assessment of renoprotective treatment in type 2 diabetic patients with microalbuminuria. BMC Nephrol. 11, 29.

169.   Caubet,C., Lacroix,C., Decramer,S., Drube,J., Ehrich,J.H., Mischak,H., Bascands,J.L., and Schanstra,J.P. (2010). Advances in urinary proteome analysis and biomarker discovery in pediatric renal disease. Pediatr. Nephrol 25, 27-35.

170.   Dakna,M., Harris,K., Kalousis,A., Carpentier,S., Kolch,W., Schanstra,J.P., Haubitz,M., Vlahou,A., Mischak,H., and Girolami,M. (2010). Addressing the Challenge of Defining Valid Proteomic Biomarkers and Classifiers. Bmc Bioinformatics 11.

171.   Delles,C., Schiffer,E., von zur Muhlen,C., Peter,K., Rossing,P., Parving,H.H., Dymott,J.A., Neisius,U., Zimmerli,L.U., Snell-Bergeon,J.K., Maahs,D.M., Schmieder,R.E., Mischak,H., and Dominiczak,A.F. (2010). Urinary proteomic diagnosis of coronary artery disease: identification and clinical validation in 623 individuals. Journal of Hypertension 28, 2316-2322.

172.   Drube,J., Zurbig,P., Schiffer,E., Lau,E., Ure,B., Gluer,S., Kirschstein,M., Pape,L., Decramer,S., Bascands,J.L., Schanstra,J.P., Mischak,H., and Ehrich,J.H.H. (2010). Urinary proteome analysis identifies infants but not older children requiring pyeloplasty. Pediatric Nephrology 25, 1673-1678.

173.   Good,D.M., Zurbig,P., Argiles,A., Bauer,H.W., Behrens,G., Coon,J.J., Dakna,M., Decramer,S., Delles,C., Dominiczak,A.F., Ehrich,J.H.H., Eitner,F., Fliser,D., Frommberger,M., Ganser,A., Girolami,M.A., Golovko,I., Gwinner,W., Haubitz,M., Herget-Rosenthal,S., Jankowski,J., Jahn,H., Jerums,G., Julian,B.A., Kellmann,M., Kliem,V., Kolch,W., Krolewski,A.S., Luppi,M., Massy,Z., Melter,M., Neususs,C., Novak,J., Peter,K., Rossing,K., Rupprecht,H., Schanstra,J.P., Schiffer,E., Stolzenburg,J.U., Tarnow,L., Theodorescu,D., Thongboonkerd,V., Vanholder,R., Weissinger,E.M., Mischak,H., and Schmitt-Kopplin,P. (2010). Naturally Occurring Human Urinary Peptides for Use in Diagnosis of Chronic Kidney Disease. Molecular & Cellular Proteomics 9, 2424-2437.

174.   Griner,E.M., Caino,M.C., Sosa,M.S., Colon-Gonzalez,F., Chalmers,M.J., Mischak,H., and Kazanietz,M.G. (2010). A novel cross-talk in diacylglycerol signaling: the Rac-GAP {beta}2-chimaerin is negatively regulated by PKC{delta} mediated phosphorylation. J Biol. Chem.

175.   Maahs,D.M., Siwy,J., Argiles,A., Cerna,M., Delles,C., Dominiczak,A.F., Gayrard,N., Iphofer,A., Jansch,L., Jerums,G., Medek,K., Mischak,H., Navis,G.J., Roob,J.M., Rossing,K., Rossing,P., Rychlik,I., Schiffer,E., Schmieder,R.E., Wascher,T.C., Winklhofer-Roob,B.M., Zimmerli,L.U., Zurbig,P., and Snell-Bergeon,J.K. (2010). Urinary Collagen Fragments Are Significantly Altered in Diabetes: A Link to Pathophysiology. Plos One 5.

176.   Metzger,J., Kirsch,T., Schiffer,E., Ulger,P., Mentes,E., Brand,K., Weissinger,E.M., Haubitz,M., Mischak,H., and Herget-Rosenthal,S. (2010). Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury. Kidney International 78, 1252-1262.

177.   Mischak,H., Delles,C., Klein,J., and Schanstra,J.P. (2010). Urinary Proteomics Based on Capillary Electrophoresis-Coupled Mass Spectrometry in Kidney Disease: Discovery and Validation of Biomarkers, and Clinical Application. Advances in Chronic Kidney Disease 17, 493-506.

178.   Mischak,H. and Rossing,P. (2010). Proteomic biomarkers in diabetic nephropathy-reality or future promise? Nephrology Dialysis Transplantation 25, 2843-2845.

179.   Mischak,H., Kolch,W., Aivaliotis,M., Bouyssie,D., Court,M., Dihazi,H., Dihazi,G.H., Franke,J., Garin,J., de Peredo,A.G., Iphoefer,A., Jänsch,L., Lacroix,C., Makridakis,M., Masselon,C., Metzger,J., Monsarrat,B., Mrug,M., Norling,M., Novak,J., Pich,A., Pitt,A., Bongcam-Rudloff,E., Siwy,J., Suzuki,H., Thongboonkerd,V., Wang,L., Zoidakis,J., Zuerbig,P., Schanstra,J., and Vlahou,A. (2010). Comprehensive human urine standards for comparability and standardization in clinical proteome analysis. Proteomics Clinical Applications 4, 464-478.

180.   Mischak,H., Allmaier,G., Apweiler,R., Attwood,T., Baumann,M., Benigni,A., Bennett,S.E., Bischoff,R., Bongcam-Rudloff,E., Capasso,G., Coon,J.J., D'Haese,P., Dominiczak,A.F., Dakna,M., Dihazi,H., Ehrich,J.H., Fernandez-Llama,P., Fliser,D., Frokiaer,J., Garin,J., Girolami,M., Hancock,W.S., Haubitz,M., Hochstrasser,D., Holman,R.R., Ioannidis,J.P., Jankowski,J., Julian,B.A., Klein,J.B., Kolch,W., Luider,T., Massy,Z., Mattes,W.B., Molina,F., Monsarrat,B., Novak,J., Peter,K., Rossing,P., Sanchez-Carbayo,M., Schanstra,J.P., Semmes,O.J., Spasovski,G., Theodorescu,D., Thongboonkerd,V., Vanholder,R., Veenstra,T.D., Weissinger,E., Yamamoto,T., and Vlahou,A. (2010). Recommendations for biomarker identification and qualification in clinical proteomics. Sci. Transl. Med. 2, 46ps42.

181.   Albalat,A., Mischak,H., and Mullen,W. (2011). Urine proteomics in clinical applications: technologies, principal considerations and clinical implementation. Prilozi. 32, 13-44.

182.   Albalat,A., Mischak,H., and Mullen,W. (2011). Clinical application of urinary proteomics/peptidomics. Expert Rev. Proteomics 8, 615-629.

183.   Carty,D.M., Siwy,J., Brennand,J.E., Zurbig,P., Mullen,W., Franke,J., McCulloch,J.W., Roberts,C.T., North,R.A., Chappell,L.C., Mischak,H., Poston,L., Dominiczak,A.F., and Delles,C. (2011). Urinary Proteomics for Prediction of Preeclampsia. Hypertension 57, 561-U387.

184.   Foucher,C., Schiffer,E., Mischak,H., Ansquer,J.C., and Wilbraham,D. (2011). Effect of fenofibrate treatment on the low molecular weight urinary proteome of healthy volunteers. Proteomics Clinical Applications 5, 159-166.

185.   Jahn,H., Wittke,S., Zurbig,P., Raedler,T.J., Arlt,S., Kellmann,M., Mullen,W., Eichenlaub,M., Mischak,H., and Wiedemann,K. (2011). Peptide fingerprinting of Alzheimer's disease in cerebrospinal fluid: identification and prospective evaluation of new synaptic biomarkers. Plos One 6, e26540.

186.   Kistler,A.D., Siwy,J., Breunig,F., Jeevaratnam,P., Scherl,A., Mullen,W., Warnock,D.G., Wanner,C., Hughes,D.A., Mischak,H., Wuthrich,R.P., and Serra,A.L. (2011). A Distinct Urinary Biomarker Pattern Characteristic of Female Fabry Patients That Mirrors Response to Enzyme Replacement Therapy. Plos One 6.

187.   Lankisch,T.O., Metzger,J., Negm,A.A., Vosskuhl,K., Schiffer,E., Siwy,J., Weismuller,T.J., Schneider,A.S., Thedieck,K., Baumeister,R., Zurbig,P., Weissinger,E.M., Manns,M.P., Mischak,H., and Wedemeyer,J. (2011). Bile Proteomic Profiles Differentiate Cholangiocarcinoma from Primary Sclerosing Cholangitis and Choledocholithiasis. Hepatology 53, 875-884.

188.   Lopez,L.M., Mullen,W., Zurbig,P., Harris,S.E., Gow,A.J., Starr,J.M., Porteous,D.J., Mischak,H., and Deary,I.J. (2011). A pilot study of urinary peptides as biomarkers for intelligence in old age. Intelligence 39, 46-53.

189.   Mayer,G., Heinze,G., Mischak,H., Hellemons,M.E., Heerspink,H.J., Bakker,S.J., de,Z.D., Haiduk,M., Rossing,P., and Oberbauer,R. (2011). Omics-bioinformatics in the context of clinical data. Methods Mol. Biol. 719, 479-497.

190.   Metzger,J., Chatzikyrkou,C., Broecker,V., Schiffer,E., Jaensch,L., Iphoefer,A., Mengel,M., Mullen,W., Mischak,H., Haller,H., and Gwinner,W. (2011). Diagnosis of subclinical and clinical acute T-cell-mediated rejection in renal transplant patients by urinary proteome analysis. Proteomics Clinical Applications 5, 322-333.

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194.   Mullen,W., Gonzalez,J., Siwy,J., Franke,J., Sattar,N., Mullan,A., Roberts,S., Delles,C., Mischak,H., and Albalat,A. (2011). A Pilot Study on the Effect of Short-Term Consumption of a Polyphenol Rich Drink on Biomarkers of Coronary Artery Disease Defined by Urinary Proteomics. J. Agric. Food Chem.

195.   Schiffer,E., Liabeuf,S., Lacroix,C., Temmar,M., Renard,C., Monsarrat,B., Choukroun,G., Lemke,H.D., Vanholder,R., Mischak,H., and Massy,Z.A. (2011). Markers of vascular disease in plasma from patients with chronic kidney disease identified by proteomic analysis. Journal of Hypertension 29, 783-790.

196.   Bandin,F., Siwy,J., Breuil,B., Mischak,H., Bascands,J.L., Decramer,S., and Schanstra,J.P. (2012). Urinary proteome analysis at 5-year followup of patients with nonoperated ureteropelvic junction obstruction suggests ongoing kidney remodeling. J Urol. 187, 1006-1011.

197.   Dawson,J., Walters,M., Delles,C., Mischak,H., and Mullen,W. (2012). Urinary proteomics to support diagnosis of stroke. Plos One 7, e35879.

198.   Drube,J., Schiffer,E., Lau,E., Petersen,C., Kirschstein,M., Kemper,M.J., Lichtinghagen,R., Ure,B., Mischak,H., Pape,L., and Ehrich,J.H. (2012). Urinary proteome analysis to exclude severe vesicoureteral reflux. Pediatrics 129, e356-e363.

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200.   Herget-Rosenthal,S., Metzger,J., Albalat,A., Bitsika,V., and Mischak,H. (2012). Proteomic biomarkers for the early detection of acute kidney injury. Prilozi 33, 27-48.

201.   Kuznetsova T., Mischak,H., Mullen,W., and Staessen,J.A. (2012). Urinary proteome analysis in hypertensive patients with left ventricular diastolic dysfuntion. Eur. Heart J. in press.

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204.   Molin,L., Seraglia,R., Lapolla,A., Ragazzi,E., Gonzalez,J., Vlahou,A., Schanstra,J.P., Albalat,A., Dakna,M., Siwy,J., Jankowski,J., Bitsika,V., Mischak,H., Zurbig,P., and Traldi,P. (2012). A comparison between MALDI-MS and CE-MS data for biomarker assessment in chronic kidney diseases. J. Proteomics 75, 5888-5897.

205.   Mullen,W., Albalat,A., Gonzalez,J., Zerefos,P., Siwy,J., Franke,J., and Mischak,H. (2012). Performance of different separation methods interfaced in the same MS-reflection TOF detector: a comparison of performance between CE versus HPLC for biomarker analysis. Electrophoresis 33, 567-574.

206.   Roscioni,S., de Zeeuw D, Hellemons,M.E., Mischak,H., Zuerbig,P., Bakker ,S.J.L., Gansevoort RT, Reinhard,H., Lajer,M., Rossing,P., and Lambers Heerspink,H.J. (2012). A urinary peptide biomarker set predicts worsening of albuminuria in type 2 diabetes. Diabetologia in press.

207.   Rouse,R., Siwy,J., Mullen,W., Mischak,H., Metzger,J., and Hanig,J. (2012). Proteomic candidate biomarkers of drug-induced nephrotoxicity in the rat. Plos One 7, e34606.

208.   Siwy,J., Zoja,C., Klein,J., Benigni,A., Mullen,W., Mayer,B., Mischak,H., Jankowski,J., Stevens,R., Vlahou,A., Kossida,S., Perco,P., and Bahlmann,F.H. (2012). Evaluation of the Zucker diabetic fatty (ZDF) rat as a model for human disease based on urinary peptidomic profiles. Plos One 7, e51334.

209.   von Zur,M.C., Schiffer,E., Sackmann,C., Zurbig,P., Neudorfer,I., Zirlik,A., Htun,N., Iphofer,A., Jansch,L., Mischak,H., Bode,C., Chen,Y.C., and Peter,K. (2012). Urine proteome analysis reflects atherosclerotic disease in an ApoE-/- mouse model and allows the discovery of new biomarkers in mouse and human atherosclerosis. Mol Cell Proteomics.

210.   Zurbig,P., Jerums,G., Hovind,P., Macisaac,R.J., Mischak,H., Nielsen,S.E., Panagiotopoulos,S., Persson,F., and Rossing,P. (2012). Urinary proteomics for early diagnosis in diabetic nephropathy. Diabetes 61 , 3304-3313.

211.   Albalat,A., Stalmach,A., Bitsika,V., Siwy,J., Schanstra,J.P., Petropoulos,A.D., Vlahou,A., Jankowski,J., Persson,F., Rossing,P., Jaskolla,T.W., Mischak,H., and Husi,H. (2013). Improving peptide relative quantification in MALDI-TOF MS for biomarker assessment. Proteomics 13, 2967-2975.

212.   Albalat,A., Franke,J., Gonzalez,J., Mischak,H., and Zurbig,P. (2013). Urinary proteomics based on capillary electrophoresis coupled to mass spectrometry in kidney disease. Methods Mol Biol 919, 203-213.

213.   Argiles,A., Siwy,J., Duranton,F., Gayrard,N., Dakna,M., Lundin,U., Osaba,L., Delles,C., Mourad,G., Weinberger,K.M., and Mischak,H. (2013). CKD273, a new proteomics classifier assessing CKD and its prognosis. Plos One 8, e62837.

214.   Dissard,R., Klein,J., Caubet,C., Breuil,B., Siwy,J., Hoffman,J., Sicard,L., Ducasse,L., Rascalou,S., Payre,B., Buleon,M., Mullen,W., Mischak,H., Tack,I., Bascands,J.L., Buffin-Meyer,B., and Schanstra,J.P. (2013). Long term metabolic syndrome induced by a high fat high fructose diet leads to minimal renal injury in C57BL/6 mice. Plos One 8, e76703.

215.   Frantzi,M., Zoidakis,J., Papadopoulos,T., Zurbig,P., Katafigiotis,I., Stravodimos,K., Lazaris,A., Giannopoulou,I., Ploumidis,A., Mischak,H., Mullen,W., and Vlahou,A. (2013). IMAC fractionation in combination with LC-MS reveals H2B and NIF-1 peptides as potential bladder cancer biomarkers. J Proteome Res 12, 3969-3979.

216.   Husi,H., Sanchez-Nino,M.D., Delles,C., Mullen,W., Vlahou,A., Ortiz,A., and Mischak,H. (2013). A combinatorial approach of Proteomics and Systems Biology in unravelling the mechanisms of acute kidney injury (AKI): involvement of NMDA receptor GRIN1 in murine AKI. BMC Syst Biol 7, 110.

217.   Jankowski,V., Schulz,A., Kretschmer,A., Mischak,H., Boehringer,F., van der,G.M., Janke,D., Schuchardt,M., Herwig,R., Zidek,W., and Jankowski,J. (2013). The enzymatic activity of the VEGFR2 receptor for the biosynthesis of dinucleoside polyphosphates. J Mol Med (Berl) 91, 1095-1107.

218.   Kistler,A.D., Serra,A.L., Siwy,J., Poster,D., Krauer,F., Torres,V.E., Mrug,M., Grantham,J.J., Bae,K.T., Bost,J.E., Mullen,W., Wuthrich,R.P., Mischak,H., and Chapman,A.B. (2013). Urinary proteomic biomarkers for diagnosis and risk stratification of autosomal dominant polycystic kidney disease: a multicentric study. Plos One 8, e53016.

219.   Klein,G., Schanstra,J.P., Hoffmann,J., Mischak,H., Siwy,J., and Zimmermann,K. (2013). Proteomics as a Quality Control Tool of Pharmaceutical Probiotic Bacterial Lysate Products. Plos One 8, e66682.

220.   Klein,J., Lacroix,C., Caubet,C., Siwy,J., Zurbig,P., Dakna,M., Muller,F., Breuil,B., Stalmach,A., Mullen,W., Mischak,H., Bandin,F., Monsarrat,B., Bascands,J.L., Decramer,S., and Schanstra,J.P. (2013). Fetal urinary peptides to predict postnatal outcome of renal disease in fetuses with posterior urethral valves (PUV). Sci Transl. Med 5, 198ra106.

221.   Klein,J., Eales,J., Zurbig,P., Vlahou,A., Mischak,H., and Stevens,R. (2013). Proteasix: a tool for automated and large-scale prediction of proteases involved in naturally occurring peptide generation. Proteomics 13, 1077-1082.

222.   Latosinska,A., Frantzi,M., Vlahou,A., and Mischak,H. (2013). Clinical applications of capillary electrophoresis coupled to mass spectrometry in biomarker discovery: Focus on Bladder Cancer. Proteomics Clin Appl.

223.   Mansor,R., Mullen,W., Albalat,A., Zerefos,P., Mischak,H., Barrett,D.C., Biggs,A., and Eckersall,P.D. (2013). A peptidomic approach to biomarker discovery for bovine mastitis. J Proteomics 85, 89-98.

224.   Metzger,J., Negm,A.A., Plentz,R.R., Weismuller,T.J., Wedemeyer,J., Karlsen,T.H., Dakna,M., Mullen,W., Mischak,H., Manns,M.P., and Lankisch,T.O. (2013). Urine proteomic analysis differentiates cholangiocarcinoma from primary sclerosing cholangitis and other benign biliary disorders. Gut 62, 122-130.

225.   Mischak,H., Vlahou,A., and Ioannidis,J.P. (2013). Technical aspects and inter-laboratory variability in native peptide profiling: the CE-MS experience. Clin Biochem. 46, 432-443.

226.   Stalmach,A., Albalat,A., Mullen,W., and Mischak,H. (2013). Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications. Electrophoresis 34, 1452-1464.

227.   Albalat,A., Husi,H., Stalmach,A., Schanstra,J.P., and Mischak,H. (2014). Classical MALDI-MS versus CE-based ESI-MS proteomic profiling in urine for clinical applications. Bioanalysis. 6, 247-266.

228.   Duranton,F., Lundin,U., Gayrard,N., Mischak,H., Aparicio,M., Mourad,G., Daures,J.P., Weinberger,K.M., and Argiles,A. (2014). Plasma and urinary amino acid metabolomic profiling in patients with different levels of kidney function. Clin. J. Am. Soc. Nephrol. 9, 37-45.

229.   Filip,S., Pontillo,C., Peter,S.J., Vlahou,A., Mischak,H., and Klein,J. (2014). Urinary proteomics and molecular determinants of chronic kidney disease: possible link to proteases. Expert Rev. Proteomics 11, 535-548.

230.   Filip,S., Zoidakis,J., Vlahou,A., and Mischak,H. (2014). Advances in urinary proteome analysis and applications in systems biology. Bioanalysis 6, 2549-2569.

231.   Frantzi,M., Metzger,J., Banks,R.E., Husi,H., Klein,J., Dakna,M., Mullen,W., Cartledge,J.J., Schanstra,J.P., Brand,K., Kuczyk,M.A., Mischak,H., Vlahou,A., Theodorescu,D., and Merseburger,A.S. (2014). Discovery and validation of urinary biomarkers for detection of renal cell carcinoma. J. Proteomics 98, 44-58.

232.   Gu,Y.M., Thijs,L., Liu,Y.P., Zhang,Z., Jacobs,L., Koeck,T., Zurbig,P., Lichtinghagen,R., Brand,K., Kuznetsova,T., Olivi,L., Verhamme,P., Delles,C., Mischak,H., and Staessen,J.A. (2014). The urinary proteome as correlate and predictor of renal function in a population study. Nephrol Dial Transplant 29, 2260-2268.

233.   Husi,H., Van,A.T., Mullen,W., Bahlmann,F.H., Schanstra,J.P., Vlahou,A., Delles,C., Perco,P., and Mischak,H. (2014). Proteome-based systems biology analysis of the diabetic mouse aorta reveals major changes in Fatty Acid biosynthesis as potential hallmark in diabetes mellitus-associated vascular disease. Circ. Cardiovasc. Genet. 7, 161-170.

234.   Klein,J., Papadopoulos,T., Mischak,H., and Mullen,W. (2014). Comparison of CE-MS/MS and LC-MS/MS sequencing demonstrates significant complementarity in natural peptide identification in human urine. Electrophoresis 35, 1060-1064.

235.   Klein,J., Buffin-Meyer,B., Mullen,W., Carty,D.M., Delles,C., Vlahou,A., Mischak,H., Decramer,S., Bascands,J.L., and Schanstra,J.P. (2014). Clinical proteomics in obstetrics and neonatology. Expert. Rev. Proteomics 11, 75-89.

236.   Koss,M.J., Hoffmann,J., Nguyen,N., Pfister,M., Mischak,H., Mullen,W., Husi,H., Rejdak,R., Koch,F., Jankowski,J., Krueger,K., Bertelmann,T., Klein,J., Schanstra,J.P., and Siwy,J. (2014). Proteomics of vitreous humor of patients with exudative age-related macular degeneration. Plos One 9, e96895.

237.   Mischak,H., Vlahou,A., Righetti,P.G., and Calvete,J.J. (2014). Putting value in biomarker research and reporting. J. Proteomics 96, A1-A3.

238.   Mullen,W., Saigusa,D., Abe,T., Adamski,J., and Mischak,H. (2014). Proteomics and Metabolomics as Tools to Unravel Novel Culprits and Mechanisms of Uremic Toxicity: Instrument or Hype? Semin. Nephrol. 34, 180-190.

239.   Nkuipou-Kenfack,E., Koeck,T., Mischak,H., Pich,A., Schanstra,J.P., Zurbig,P., and Schumacher,B. (2014). Proteome analysis in the assessment of ageing. Ageing Res Rev. 18C, 74-85.

240.   Nkuipou-Kenfack,E., Duranton,F., Gayrard,N., Argiles,A., Lundin,U., Weinberger,K.M., Dakna,M., Delles,C., Mullen,W., Husi,H., Klein,J., Koeck,T., Zurbig,P., and Mischak,H. (2014). Assessment of metabolomic and proteomic biomarkers in detection and prognosis of progression of renal function in chronic kidney disease. Plos One 9, e96955.

241.   Rodriguez-Suarez,E., Siwy,J., Zurbig,P., and Mischak,H. (2014). Urine as a source for clinical proteome analysis: From discovery to clinical application. Biochim. Biophys. Acta 1844, 884-898.

242.   Schanstra,J.P. and Mischak,H. (2014). Proteomic urinary biomarker approach in renal disease: from discovery to implementation. Pediatr. Nephrol.

243.   Seetho,I.W., Siwy,J., Albalat,A., Mullen,W., Mischak,H., Parker,R.J., Craig,S., Duffy,N., Hardy,K.J., Burniston,J.G., and Wilding,J.P. (2014). Urinary proteomics in obstructive sleep apnoea and obesity. Eur. J Clin Invest 44, 1104-1115.

244.   Siwy,J., Schanstra,J.P., Argiles,A., Bakker,S.J., Beige,J., Boucek,P., Brand,K., Delles,C., Duranton,F., Fernandez-Fernandez,B., Jankowski,M.L., Al,K.M., Kunt,T., Lajer,M., Lichtinghagen,R., Lindhardt,M., Maahs,D.M., Mischak,H., Mullen,W., Navis,G., Noutsou,M., Ortiz,A., Persson,F., Petrie,J.R., Roob,J.M., Rossing,P., Ruggenenti,P., Rychlik,I., Serra,A.L., Snell-Bergeon,J., Spasovski,G., Stojceva-Taneva,O., Trillini,M., von der,L.H., Winklhofer-Roob,B.M., Zurbig,P., and Jankowski,J. (2014). Multicentre prospective validation of a urinary peptidome-based classifier for the diagnosis of type 2 diabetic nephropathy. Nephrol. Dial. Transplant.

245.   Stalmach,A., Johnsson,H., McInnes,I.B., Husi,H., Klein,J., Dakna,M., Mullen,W., Mischak,H., and Porter,D. (2014). Identification of urinary peptide biomarkers associated with rheumatoid arthritis. Plos One 9, e104625.

246.   Weissinger,E.M., Metzger,J., Dobbelstein,C., Wolff,D., Schleuning,M., Kuzmina,Z., Greinix,H., Dickinson,A.M., Mullen,W., Kreipe,H., Hamwi,I., Morgan,M., Krons,A., Tchebotarenko,I., Ihlenburg-Schwarz,D., Dammann,E., Collin,M., Ehrlich,S., Diedrich,H., Stadler,M., Eder,M., Holler,E., Mischak,H., Krauter,J., and Ganser,A. (2014). Proteomic peptide profiling for preemptive diagnosis of acute graft-versus-host disease after allogeneic stem cell transplantation. Leukemia 28, 842-852.

247.   Zhang,Z., Staessen,J.A., Thijs,L., Gu,Y., Liu,Y., Jacobs,L., Koeck,T., Zurbig,P., Mischak,H., and Kuznetsova,T. (2014). Left ventricular diastolic function in relation to the urinary proteome: a proof-of-concept study in a general population. Int. J Cardiol. 176, 158-165.

248.   Bhat,A., Dakna,M., and Mischak,H. (2015). Integrating proteomics profiling data sets: a network perspective. Methods Mol Biol 1243, 237-253.

249.   Bhat,A., Heinzel,A., Mayer,B., Perco,P., Muhlberger,I., Husi,H., Merseburger,A.S., Zoidakis,J., Vlahou,A., Schanstra,J.P., Mischak,H., and Jankowski,V. (2015). Protein interactome of muscle invasive bladder cancer. Plos One 10, e0116404.

250.   Brown,C.E., McCarthy,N.S., Hughes,A.D., Sever,P., Stalmach,A., Mullen,W., Dominiczak,A.F., Sattar,N., Mischak,H., Thom,S., Mayet,J., Stanton,A.V., and Delles,C. (2015). Urinary proteomic biomarkers to predict cardiovascular events. Proteomics Clin Appl.

251.   Filip,S., Vougas,K., Zoidakis,J., Latosinska,A., Mullen,W., Spasovski,G., Mischak,H., Vlahou,A., and Jankowski,J. (2015). Comparison of Depletion Strategies for the Enrichment of Low-Abundance Proteins in Urine. PLoS One 10, e0133773.

252.   Frantzi,M., Latosinska,A., Fluhe,L., Hupe,M.C., Critselis,E., Kramer,M.W., Merseburger,A.S., Mischak,H., and Vlahou,A. (2015a). Developing proteomic biomarkers for bladder cancer: towards clinical application. Nat. Rev. Urol 12, 317-330.

253.   Frantzi,M., Latosinska,A., Merseburger,A.S., and Mischak,H. (2015b). Recent progress in urinary proteome analysis for prostate cancer diagnosis and management. Expert Rev. Mol. Diagn. 15, 1539-1554.

254.   Gleiss,A., Dakna,M., Mischak,H., and Heinze,G. (2015). Two-group comparisons of zero-inflated intensity values: the choice of test statistic matters. Bioinformatics. 31, 2310-2317.

255.   Glorieux,G., Mullen,W., Duranton,F., Filip,S., Gayrard,N., Husi,H., Schepers,E., Neirynck,N., Schanstra,J.P., Jankowski,J., Mischak,H., Argiles,A., Vanholder,R., Vlahou,A., and Klein,J. (2015). New insights in molecular mechanisms involved in chronic kidney disease using high-resolution plasma proteome analysis. Nephrol. Dial. Transplant. 30, 1842-1852.

256.   Jankowski,J., Schanstra,J.P., and Mischak,H. (2015). Body fluid peptide and protein signatures in diabetic kidney diseases. Nephrol. Dial. Transplant. 30 Suppl 4, iv43-iv53.

257.   Latosinska,A., Vougas,K., Makridakis,M., Klein,J., Mullen,W., Abbas,M., Stravodimos,K., Katafigiotis,I., Merseburger,A.S., Zoidakis,J., Mischak,H., Vlahou,A., and Jankowski,V. (2015). Comparative Analysis of Label-Free and 8-Plex iTRAQ Approach for Quantitative Tissue Proteomic Analysis. PLoS One 10, e0137048.

  1. Mischak,H., Critselis,E., Hanash,S., Gallagher,W.M., Vlahou,A., and Ioannidis,J.P. (2015). Epidemiologic design and analysis for proteomic studies: a primer on -omic technologies. Am. J Epidemiol. 181, 635-647.
  2. Mischak,H., Delles,C., Vlahou,A., and Vanholder,R. (2015). Proteomic biomarkers in kidney disease: issues in development and implementation. Nat. Rev. Nephrol 11, 221-232.
  3. Mischak,H. (2015). Pro: Urine proteomics as a liquid kidney biopsy: no more kidney punctures! Nephrol Dial Transplant 30, 532-537.
  4. Mischak,H. (2015). Opponent's comments. Nephrol Dial Transplant 30, 531-532.
  5. Nally,J.E., Mullen,W., Callanan,J.J., Mischak,H., and Albalat,A. (2015). Detection of urinary biomarkers in reservoir hosts of leptospirosis by capillary electrophoresis-mass spectrometry. Proteomics Clin. Appl. 9, 543-551.
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  7. Pejchinovski,M., Hrnjez,D., Ramirez-Torres,A., Bitsika,V., Mermelekas,G., Vlahou,A., Zurbig,P., Mischak,H., Metzger,J., and Koeck,T. (2015). Capillary zone electrophoresis on-line coupled to mass spectrometry: A perspective application for clinical proteomics. Proteomics Clin. Appl. 9, 453-468.
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  9. Pena,M.J., de,Z.D., Mischak,H., Jankowski,J., Oberbauer,R., Woloszczuk,W., Benner,J., Dallmann,G., Mayer,B., Mayer,G., Rossing,P., and Lambers Heerspink,H.J. (2015). Prognostic clinical and molecular biomarkers of renal disease in type 2 diabetes. Nephrol. Dial. Transplant. 30 Suppl 4, iv86-iv95.
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  11. Schanstra,J.P., Zurbig,P., Alkhalaf,A., Argiles,A., Bakker,S.J., Beige,J., Bilo,H.J., Chatzikyrkou,C., Dakna,M., Dawson,J., Delles,C., Haller,H., Haubitz,M., Husi,H., Jankowski,J., Jerums,G., Kleefstra,N., Kuznetsova,T., Maahs,D.M., Menne,J., Mullen,W., Ortiz,A., Persson,F., Rossing,P., Ruggenenti,P., Rychlik,I., Serra,A.L., Siwy,J., Snell-Bergeon,J., Spasovski,G., Staessen,J.A., Vlahou,A., Mischak,H., and Vanholder,R. (2015). Diagnosis and Prediction of CKD Progression by Assessment of Urinary Peptides. J Am Soc. Nephrol. 26, 1999-2010.
  12. Seetho,I.W., Ramirez-Torres,A., Albalat,A., Mullen,W., Mischak,H., Parker,R.J., Craig,S., Duffy,N., Hardy,K.J., Burniston,J.G., and Wilding,J.P. (2015). Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment. Sleep Sci. 8, 58-67.

270.   Silva,S., Bronze,M.R., Figueira,M.E., Siwy,J., Mischak,H., Combet,E., and Mullen,W. (2015). Impact of a 6-wk olive oil supplementation in healthy adults on urinary proteomic biomarkers of coronary artery disease, chronic kidney disease, and diabetes (types 1 and 2): a randomized, parallel, controlled, double-blind study. Am. J Clin Nutr. 101, 44-54.

271.   Stalmach,A., Husi,H., Mosbahi,K., Albalat,A., Mullen,W., and Mischak,H. (2015). Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids. Methods Mol Biol 1243, 187-205.

272.   Zhang,Z.Y., Thijs,L., Petit,T., Gu,Y.M., Jacobs,L., Yang,W.Y., Liu,Y.P., Koeck,T., Zurbig,P., Jin,Y., Verhamme,P., Voigt,J.U., Kuznetsova,T., Mischak,H., and Staessen,J.A. (2015). Urinary Proteome and Systolic Blood Pressure as Predictors of 5-Year Cardiovascular and Cardiac Outcomes in a General Population. Hypertension 66, 52-60.

273.   Cisek,K., Krochmal,M., Klein,J., and Mischak,H. (2015). The application of multi-omics and systems biology to identify therapeutic targets in chronic kidney disease. Nephrol. Dial. Transplant., 2003-2011.

274.   Klein,J., Bascands,J.L., Mischak,H., and Schanstra,J.P. (2016). The role of urinary peptidomics in kidney disease research. Kidney Int. 89, 539-545.

275.   Schonemeier,B., Metzger,J., Klein,J., Husi,H., Bremer,B., Armbrecht,N., Dakna,M., Schanstra,J.P., Rosendahl,J., Wiegand,J., Jager,M., Mullen,W., Breuil,B., Plentz,R.R., Lichtinghagen,R., Brand,K., Kuhnel,F., Mischak,H., Manns,M.P., and Lankisch,T.O. (2016). Urinary Peptide Analysis Differentiates Pancreatic Cancer From Chronic Pancreatitis. Pancreas.

276.   Drube,J., Zuerbig,P., Mischak,H., and Pape,L. (2016). Chronic kidney disease can be identified reliably by urinary proteome analysis in children after orthotopic liver transplantation. Pediatric Nephrology 31, 1748.

277.   Frantzi,M., van Kessel,K.E., Zwarthoff,E., Marquez,M., Raya,M., Malats,N., Merseburger,A.S., Katafigiotis,I., Stravodimos,K., Mullen,W., Zoidakis,J., Makridakis,M., Pejchinovski,M., Critselis,E., Lichtinghagen,R., Brand,K., Dakna,M., Roubelakis,M.G., Theodorescu,D., Vlahou,A., Mischak,H., and Anagnou,N.P. (2016). Development and validation of urine-based peptide biomarker panels for detecting bladder cancer in a multi-center study. Clinical Cancer Research 22: 4077-4086.

278.   Frantzi,M., Klimou,Z., Makridakis,M., Zoidakis,J., Latosinska,A., Borras,D.M., Janssen,B., Giannopoulou,I., Lygirou,V., Lazaris,A.C., Anagnou,N.P., Mischak,H., Roubelakis,M.G., and Vlahou,A. (2016). Silencing of Profilin-1 suppresses cell adhesion and tumor growth via predicted alterations in integrin and Ca2+ signaling in T24M-based bladder cancer models. Oncotarget 7, 70750-70768.

279.   Pena MJ, Mischak H, Heerspink HJ (2016) Proteomics for prediction of disease progression and response to therapy in diabetic kidney disease. Diabetologia 59: 1819-1831.

280.   Lindhardt M, Persson F, Zurbig P, Stalmach A, Mischak H et al. (2016) Urinary proteomics predict onset of microalbuminuria in normoalbuminuric type 2 diabetic patients, a sub-study of the DIRECT-Protect 2 study. Nephrol Dial Transplant in press:

281.   Latosinska,A., Makridakis,M., Frantzi,M., Borras,D.M., Janssen,B., Mullen,W., Zoidakis,J., Merseburger,A.S., Jankowski,V., Mischak,H., and Vlahou,A. (2016). Integrative analysis of extracellular and intracellular bladder cancer cell line proteome with transcriptome: improving coverage and validity of -omics findings. Scientific Reports 6.

282.   Lindhardt,M., Persson,F., Currie,G., Pontillo,C., Beige,J., Delles,C., von der,L.H., Mischak,H., Navis,G., Noutsou,M., Ortiz,A., Ruggenenti,P.L., Rychlik,I., Spasovski,G., and Rossing,P (2016) Proteomic prediction and Renin angiotensin aldosterone system Inhibition prevention Of early diabetic nephRopathy in TYpe 2 diabetic patients with normoalbuminuria (PRIORITY): essential study design and rationale of a randomised clinical multicentre trial. BMJ Open 6: e010310.

283.   Magalhaes,P., Mischak,H., and Zuerbig,P. (2016a). Urinary proteomics using capillary electrophoresis coupled to mass spectrometry for diagnosis and prognosis in kidney diseases. Current Opinion in Nephrology and Hypertension 25, 494-501.

284.   Magalhaes,P., Schanstra,J.P., Carrick,E., Mischak,H., and Zuerbig,P. (2016b). Urinary biomarkers for renal tract malformations. Expert Review of Proteomics 13, 1121-1129.

285.   Martin-Pozuelo,G., Gonzalez-Barrio,R., Barbera,G.G., Albalat,A., Garcia-Alonso,J., Mullen,W., Mischak,H., and Periago,M.J. (2016). Tomato Juice Consumption Modifies the Urinary Peptide Profile in Sprague-Dawley Rats with Induced Hepatic Steatosis. International Journal of Molecular Sciences 17.

286.   Metzger,J., Mullen,W., Husi,H., Stalmach,A., Herget-Rosenthal,S., Groesdonk,H.V., Mischak,H., and Klingele,M. (2016). Acute kidney injury prediction in cardiac surgery patients by a urinary peptide pattern: a case-control validation study. Critical Care 20.

287.   Stepczynska A, Schanstra JP, Mischak H (2016) Implementation of CE-MS-identified proteome-based biomarker panels in drug development and patient management. Bioanalysis 8: 439-455.

288.   Klein,J., Ramirez-Torres,A., Ericsson,A., Huang,Y., Breuil,B., Siwy,J., Mischak,H., Peng,X.R., Bascands,J.L., and Schanstra,J.P. (2016) Urinary peptidomics provides a noninvasive humanized readout of diabetic nephropathy in mice. Kidney Int in press:

289.   Lindhardt M, Persson FI, Oxlund C., Jacobsen IA, Zuerbig,P., Mischak H, Rossing P, and Heerspink,H.J.L. (2016) Predicting albuminuria response to spironolactone treatment with urinary proteomics in patients with type 2 diabetes and hypertension. Nephrol Dial Transplant in press:

290.   Pontillo,C., Jacobs,L., Staessen,J., Schanstra,J.P., Rossing,P., Heerspink,H.J.L., Siwy,J., Mullen,W., Vlahou,A., Mischak,H., Vanholder,R., Zürbig,P., and Jankowski,J. (2016) A urinary proteome-based classifier for the early detection of decline in glomerular filtration. Nephrol Dial Transplant Epub ahead of print (gfw239):

291.   Pontillo,C. and Mischak,H. (2016). Urinary biomarkers to predict CKD: is the future in multi-marker panels? Nephrology Dialysis Transplantation 31, 1373-1375.

292.   Siwy,J., Zürbig,P., Argiles,A., Beige,J., Haubitz,M., Jankowski,J., Julian,B.A., Linde,P.G., Marx,D., Mischak,H., Mullen,W., Novak,J., Ortiz,A., Persson,F., Pontillo,C., Rossing,P., Rupprecht,H., Schanstra,J.P., Vlahou,A., and Vanholder,R (2016) Non-invasive diagnosis of chronic kidney diseases using urinary proteome analysis. Nephrol Dial Transplant in press:

293.   Carrick,E., Vanmassenhove,J., Glorieux,G., Metzger,J., Dakna,M., Pejchinovski,M., Jankowski,V., Mansoorian,B., Husi,H., Mullen,W., Mischak,H., Vanholder,R., and Van,B.W. (2016) Development of a MALDI MS-based platform for early detection of acute kidney injury. Proteomics Clin Appl

294.   Farmakis,D., Koeck,T., Mullen,W., Parissis,J., Gogas,B.D., Nikolaou,M., Lekakis,J., Mischak,H., and Filippatos,G.. (2016) Urine proteome analysis in heart failure with reduced ejection fraction complicated by chronic kidney disease: feasibility, and clinical and pathogenetic correlates. Eur J Heart Fail 18: 822-829.

295.   Rossing,K., Bosselmann,H.S., Gustafsson,F., Zhang,Z.Y., Gu,Y.M., Kuznetsova,T., Nkuipou-Kenfack,E., Mischak,H., Staessen,J.A., Koeck,T., and Schou,M. (2016) Urinary Proteomics Pilot Study for Biomarker Discovery and Diagnosis in Heart Failure with Reduced Ejection Fraction. PLoS ONE 11: e0157167.

296.   . Pejchinovski M, Siwy J, Metzger J, Dakna M, Mischak H et al. (2017) Urine peptidome analysis predicts risk of end-stage renal disease and reveals proteolytic pathways involved in autosomal dominant polycystic kidney disease progression. Nephrol Dial Transplant 32: 487-497.

297.   Lindhardt M, Persson F, Zurbig P, Stalmach A, Mischak H et al. (2016) Urinary proteomics predict onset of microalbuminuria in normoalbuminuric type 2 diabetic patients, a sub-study of the DIRECT-Protect 2 study. Nephrol Dial Transplant in press

298.   Krochmal,M., Fernandes,M., Filip,S., Pontillo,C., Husi,H., Zoidakis,J., Mischak,H., Vlahou,A., and Jankowski,J. (2016) PeptiCKDdb-peptide- and protein-centric database for the investigation of genesis and progression of chronic kidney disease. Database, in press

299.   Stanley,E., Delatola,E.I., Nkuipou-Kenfack,E., Spooner,W., Kolch,W., Schanstra,J.P., Mischak,H., and Koeck,T. (2016). Comparison of different statistical approaches for urinary peptide biomarker detection in the context of coronary artery disease. Bmc Bioinformatics 17.

300.   von zur Muehlen,C., Koeck,T., Schiffer,E., Sackmann,C., Zuerbig,P., Hilgendorf,I., Reinoehl,J., Rivera,J., Zirlik,A., Hehrlein,C., Mischak,H., Bode,C., and Peter,K. (2016). Urine proteome analysis as a discovery tool in patients with deep vein thrombosis and pulmonary embolism. Proteomics Clinical Applications 10, 574-584.

301.   Zhang,Z.Y., Ravassa,S., Yang,W.Y., Petit,T., Pejchinovski,M., Zuerbig,P., Lopez,B., Wei,F.F., Pontillo,C., Thijs,L., Jacobs,L., Gonzalez,A., Koeck,T., Delles,C., Voigt,J.U., Verhamme,P., Kuznetsova,T., Diez,J., Mischak,H., and Staessen,J.A. (2016). Diastolic Left Ventricular Function in Relation to Urinary and Serum Collagen Biomarkers in a General Population. Plos One 11.

302.   Albalat,A., Mullen,W., Husi,H., and Mischak,H. (2017). Tissue Proteomics in Vascular Disease. Methods in molecular biology (Clifton, N. J. ) 1527, 53-60.

303.   Carleo,A., Chorostowska-Wynimko,J., Koeck,T., Mischak,H., Czajkowska-Malinowska,M., Rozy,A., Welte,T., and Janciauskiene,S. (2017). Does urinary peptide content differ between COPD patients with and without inherited alpha-1 antitrypsin deficiency? International Journal of Chronic Obstructive Pulmonary Disease 12, 829-837.

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