R&D Areas | Transplantation | Graft-versus-Host Disease

Acute Graft-versus-Host Disease (aGvHD) contributes significantly to morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Diagnosis of GvHD is mainly based on clinical features and tissue biopsies.

Mosaiques developed an application of capillary electrophoresis coupled online with mass spectrometry (CE-MS) to detect about 170 GvHDspecific polypeptides. A tentatively aGvHD-specific model consisting of 31 polypeptides was chosen, allowing correct classification of 13 of 13 (sensitivity 100.0%) aGvHD samples and 49 of 50 (specificity 98.0%) control samples of the training set. The subsequent blinded evaluation of 599 samples enabled diagnosis of aGvHD greater than grade II, even prior to clinical diagnosis, with a sensitivity of 83.1% and a specificity of 75.6%.